Protein Info for ABIE41_RS14085 in Bosea sp. OAE506

Annotation: threonine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 669 PF02824: TGS" amino acids 6 to 62 (57 residues), 28.3 bits, see alignment 2.9e-10 TIGR00418: threonine--tRNA ligase" amino acids 73 to 648 (576 residues), 659.8 bits, see alignment E=2.3e-202 PF07973: tRNA_SAD" amino acids 172 to 220 (49 residues), 46 bits, see alignment 8.5e-16 PF00587: tRNA-synt_2b" amino acids 336 to 548 (213 residues), 155.7 bits, see alignment E=2.8e-49 PF03129: HGTP_anticodon" amino acids 560 to 647 (88 residues), 64.2 bits, see alignment E=2e-21

Best Hits

Swiss-Prot: 76% identical to SYT_RHOPA: Threonine--tRNA ligase (thrS) from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)

KEGG orthology group: K01868, threonyl-tRNA synthetase [EC: 6.1.1.3] (inferred from 75% identity to xau:Xaut_1638)

Predicted SEED Role

"Threonyl-tRNA synthetase (EC 6.1.1.3)" (EC 6.1.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (669 amino acids)

>ABIE41_RS14085 threonine--tRNA ligase (Bosea sp. OAE506)
MTISLTFPDGAKREFPSGITGKEIAGGISPSLLKRTVAMAIDGVVVDLADPIGKDSKIEF
LTREDPRALELIRHDCAHVLAEAVQALWPGTQVTIGPVIEGGFFYDFARNEPFTPDDLPV
IEKKMREIIARDAPFTKDEWSRDKAKAFFAAKGEAYKVELVDAIPEDQTLKMYAQGEWID
LCRGPHMTSVGKVGTAFKLMKVAGAYWRGDSNNAMLTRIYGTAFAKQEQLDAHLHQIEEA
EKRDHRKLGREMNLFHFQEEGPGVVFWHSKGWRLFQEILTYMRRRLAADYEEVNAPQILD
KSLWETSGHWGWYQDNMFAVKSASAFENPNDATKDQKVFALKPMNCPGHVQIFKHGLKSY
RDLPIRLAEFGAVHRYEPSGALHGLMRVRGFTQDDAHIFCTEDQLAAECLKINDLILSVY
EDFGFTDDLVIKLSTRPEKRVGSDALWDHAEDVMNRVLIEIAAQSGGRIKTEINPGEGAF
YGPKFEYVLRDAIGRDWQCGTTQVDFNLPERFGAFYIGADGEKKQPVMVHRAICGSLERF
TGILIENFAGHFPLWLAPEQILVCPITSEADAYAEDVTMACLSAGLRARADLRNEKISYK
VREHSLAKVPVILAVGKREAEERTVTVRRLGSQAQTVMSLDAMLEAMLDEATPPDLKRVK
AARAAKAAG