Protein Info for ABIE41_RS12580 in Bosea sp. OAE506

Annotation: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01225: Mur_ligase
  • amino acids 27 to 80 (54 residues), 37.5 bits, 3.8e-13
• TIGR01143: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
  • amino acids 32 to 463 (432 residues), 342.8 bits, E=1.4e-106
• PF08245: Mur_ligase_M
  • amino acids 112 to 302 (191 residues), 124.2 bits, E=1.1e-39

Best Hits

KEGG orthology group: K01929, UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase [EC: 6.3.2.10] (inferred from 57% identity to rpa:RPA3534)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.10)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• Lysine biosynthesis
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.10

Protein Sequence (474 amino acids)

>ABIE41_RS12580 UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanine ligase (Bosea sp. OAE506)
MSAPLWSGDELVAALGARAEGPVPAAISGISIDTRTLEPGDAFFAITGEARNGHDFVATA
LEKGAALVVVDEAHAAQFAGKGALAIVPDVLKAMERAGSVRRAELKAGVLAVTGSVGKTG
TKEALRLILSRQGKTHAPVASYNNHWGVPLTLCRTPRDVAYAVYEIGMNHPGEILPLARL
VRPQVSIITTIQPVHLEAFASLEGIAEEKAGVFWELEPGGTAILNADIPQADLLERIARA
GPAGEVIRFGESADADVRLVSCALRSDVSTVDAVVMGQPITYRLGSPGKHIVLNSLAVLA
AVKAVGADIALAALALGDLRPPAGRGAQQRLEAPGGAFTLIDESYNGNPASVKAAIENLG
RIPVAGRGRRIAVLGDMLELGPTGPDLHKGLGPAITANGIDSVFACGPLMRGLYDSLPST
LRGAYAAQASGLEPLVLDAVRAGDVVMVKGSLGSRMGPIVKALTTRFPTLSAED