Protein Info for ABIE41_RS12250 in Bosea sp. OAE506
Annotation: AtzE family amidohydrolase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 56% identical to ATZE_PSESD: 1-carboxybiuret hydrolase subunit AtzE (atzE) from Pseudomonas sp. (strain ADP)
KEGG orthology group: K02433, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A [EC: 6.3.5.6 6.3.5.7] (inferred from 71% identity to azl:AZL_a09080)MetaCyc: 56% identical to 1-carboxybiuret hydrolase catalytic subunit (Pseudomonas sp. ADP)
Omega-amidase. [EC: 3.5.1.131, 3.5.1.3]; 3.5.1.131 [EC: 3.5.1.131, 3.5.1.3]; 3.5.1.131 [EC: 3.5.1.131, 3.5.1.3]; 3.5.1.131 [EC: 3.5.1.131, 3.5.1.3]
Predicted SEED Role
"Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases"
MetaCyc Pathways
- L-citrulline biosynthesis (7/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- superpathway of L-citrulline metabolism (9/12 steps found)
- L-glutamine degradation I (1/1 steps found)
- cyanuric acid degradation I (3/5 steps found)
- L-glutamate and L-glutamine biosynthesis (4/7 steps found)
- L-methionine salvage cycle III (5/11 steps found)
- S-methyl-5-thio-α-D-ribose 1-phosphate degradation I (2/7 steps found)
- L-methionine salvage cycle I (bacteria and plants) (4/12 steps found)
- L-methionine salvage cycle II (plants) (3/11 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.5.6, 6.3.5.7
Use Curated BLAST to search for 3.5.1.131 or 3.5.1.3 or 6.3.5.6 or 6.3.5.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (464 amino acids)
>ABIE41_RS12250 AtzE family amidohydrolase (Bosea sp. OAE506) MTLDVFTPAHRIASQVREGEVTARAVTEAFLARIAALDSAINAFTDVTAERALAQASAID AARAAGAPLGPLAGVPFAAKNLFDIAGLPTRAGSKINRERKPAARDAVLVERLTAAGAVL LGGLNMGEYAYDFTGENAHDGPCRNPHDPARMAGGSSSGSGAATAAGFSPLSLGSDTNGS IRVPSSLCGVFGLKPTYGRLPRTRSFPFCDSLDHLGPFARDVTDLATAYDALQGPDAHDP ACAQRPVEPVLPSLQAGVGELRIAVAGGHFATEGMPEAAAAVARVAQALGATQHVALEGA AIARAAAFLITNSESATFHLDRLRKRADDFDPETRDRFLAGAMLPAAWYVQAQRARRWFH DEMMRVFADVDLIIAPATPCPAPLVGQKTLELRGETVPLRPNLGLFTQPISAIGLPVAAV PVFGAGLPIGVQIIAAPWREDLCLRAAFMLQRDGVCEARAPVLG