Protein Info for ABIE41_RS09555 in Bosea sp. OAE506

Annotation: tRNA lysidine(34) synthetase TilS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02432: tRNA(Ile)-lysidine synthetase
  • amino acids 31 to 211 (181 residues), 166.1 bits, E=4.2e-53
• PF01171: ATP_bind_3
  • amino acids 32 to 210 (179 residues), 170.7 bits, E=1.5e-54

Best Hits

Swiss-Prot: 52% identical to TILS_METS4: tRNA(Ile)-lysidine synthase (tilS) from Methylobacterium sp. (strain 4-46)

KEGG orthology group: K04075, tRNA(Ile)-lysidine synthase [EC: 6.3.4.-] (inferred from 52% identity to xau:Xaut_3082)

Predicted SEED Role

"tRNA(Ile)-lysidine synthetase (EC 6.3.4.19)" (EC 6.3.4.19)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.- or 6.3.4.19

Protein Sequence (351 amino acids)

>ABIE41_RS09555 tRNA lysidine(34) synthetase TilS (Bosea sp. OAE506)
MSAVEPLSNDYPPIGACEATPLFADLAGEPRLLVAVSGGPDSVALLALLADWAREPGRPL
LAAATVDHGLRAASADEAVAVAALCARLAIPHRILHWEGNKPSSRLQERARAARYALLAQ
EAERIGGAVLVTAHTRDDQAETLLMRMARGSGPSGLAGMRTRVMRHGMALARPLLGLPKA
RLIATAQARGLPFVTDPSNGDPKFARARWRALMPQLAAEGLDAERLSLLAGRLARLDEAV
SARAQAIADAQLSAEPGAPLLRLNFEALRQEPEEIVLRVVALAIARLDVEAEGAVRLERL
EAFVSALLEAAQAGLPLTRTLAGTVLTLRRDGALSLRPEGPRRRGIHPASS