Protein Info for ABIE41_RS08145 in Bosea sp. OAE506

Annotation: CoA ester lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03328: HpcH_HpaI
  • amino acids 8 to 226 (219 residues), 151.3 bits, E=4e-48
• PF15617: C-C_Bond_Lyase
  • amino acids 10 to 290 (281 residues), 44.3 bits, E=2.1e-15

Best Hits

Swiss-Prot: 52% identical to MCTE_RHOS4: (3S)-malyl-CoA thioesterase (mcl2) from Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)

KEGG orthology group: K01644, citrate lyase subunit beta / citryl-CoA lyase [EC: 4.1.3.34 4.1.3.6] (inferred from 66% identity to xau:Xaut_1974)

Predicted SEED Role

"L-malyl-CoA/beta-methylmalyl-CoA lyase (EC 4.1.3.-)" (EC 4.1.3.-)

MetaCyc Pathways

• citrate degradation (2/2 steps found)
• reductive TCA cycle II (6/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Citrate cycle (TCA cycle)
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.-, 4.1.3.34, 4.1.3.6

Use Curated BLAST to search for 4.1.3.- or 4.1.3.34 or 4.1.3.6

Protein Sequence (291 amino acids)

>ABIE41_RS08145 CoA ester lyase (Bosea sp. OAE506)
MTTIRPRRSVLYMPGSNARALEKAREIAADALILDMEDAVAPEAKAAAREQVCAAVKAGG
YGRRELVIRTNGVDTEWFRDDLGAAVEANPDAILIPKVSTPETLEQIGAQLDGLWAQPAL
QVWAMIETPLAILHAEAIARAARDPRSRLSCFVMGTNDLAKETRARFVPGRAPMLPWLTS
AILAARAHGLDILDGVYNDLKNEEGFLAECEQARDLGFDGKTLIHPAQVGTANAVFAPDE
AELAQAKAIIAAFALPENQGKGAIQLGGRMVELLHAEMAKRVVALAEAIGD