Protein Info for ABIE41_RS07915 in Bosea sp. OAE506

Annotation: bifunctional proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase PutA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1030 transmembrane" amino acids 686 to 702 (17 residues), see Phobius details PF14850: Pro_dh-DNA_bdg" amino acids 58 to 167 (110 residues), 139.2 bits, see alignment E=1.2e-44 PF01619: Pro_dh" amino acids 177 to 469 (293 residues), 338.2 bits, see alignment E=6.8e-105 TIGR01238: delta-1-pyrroline-5-carboxylate dehydrogenase" amino acids 505 to 1008 (504 residues), 654 bits, see alignment E=6.8e-201 PF00171: Aldedh" amino acids 557 to 1012 (456 residues), 384.5 bits, see alignment E=9.4e-119

Best Hits

KEGG orthology group: K13821, proline dehydrogenase / delta 1-pyrroline-5-carboxylate dehydrogenase [EC: 1.5.1.12 1.5.99.8] (inferred from 69% identity to met:M446_2202)

Predicted SEED Role

"Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)" in subsystem Arginine and Ornithine Degradation or Proline, 4-hydroxyproline uptake and utilization or Respiratory dehydrogenases 1 (EC 1.5.1.12, EC 1.5.99.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.12 or 1.5.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1030 amino acids)

>ABIE41_RS07915 bifunctional proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase PutA (Bosea sp. OAE506)
MAAPLPPFRAPFAQDDGEIAAQLLAGARREPAAKARIDARATELIEAIRSRKVGLGGIEE
LLREYSLSTREGLALMVLAEALLRVPDAATADRLIEDKLGQGDFAHHESKSDAFLVSASA
WALGITARVIQPGETPTSIIGGLAKRLGMPTVRTATRQAMRVMGNHFVLGQTIEEALKRA
GSKSGKLYRYSFDMLGEGARTQADADRYFASYAAAIDAIGRSAGNAPLPNRPGISVKLSA
LHPRYDATNRERVLAELTPKVIALAQQAKRYDLNFTVDAEEADRLELSLDIIDAVVADPS
LADWDGFGLAIQAYQKRASAVIAHIGALAEAHGRRMMVRLVKGAYWDTELKRAQERGLPD
YPVFTRKAMTDLNYEACAAQLLALRPLIIPQFATHNAQTVATVAEMAGSAEGFEFQRLHG
MGEALYEKLIAGSEGFACRTYAPVGGHQDLLAYLVRRLLENGANSSFVSVSGDPDVPVAQ
LLVPPADIIGQPGAARHRRIPLPADLFGPSRKNSAGVELGHAESLEALLAEIAAAAGKPF
PEATPIIDGAPAGGSSRDIRSPIDNHVIGRVTEADAAIAARAMAAAKAGFPAWNATPART
RAAALRKAADLMEARRGLLLALLQAEAGKTLDDALSEVREAVDFCRYYAAEAEAKLSTPT
ALPGPTGEDNRLILRGRGAFLCIAPWNFPLAIFAGQIVAALVAGNSVVAKPAPQTPLIAA
VTVRLLHEAGIPASALHLVPGDGAVGAALVAHKDVAGVAFTGSTTTARAINRTLAAKDGP
IVPLIAETGGLNAMIVDATALAEQVADDVVMSAFRSAGQRCSALRLLVVQDDVADKMVEM
ITGAARELKLGDPRAIDTHIGPVIDAAAKHRLDGHIDAMRKLGRVAWAGQAPALGGAYVA
PHVVTLGSVADLSQEHFGPILHVVRWKAGELDRVIHDIEATGYGLTLGVHSRIETTIEQV
TSRLSTGNIYVNRNIIGAVVGVQPFGGHGLSGTGPKAGGPHYLMHFATEQTVTINTAAAG
GNASLIAMGE