Protein Info for ABIE41_RS07700 in Bosea sp. OAE506

Annotation: alpha/beta fold hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00561: Abhydrolase_1
  • amino acids 66 to 213 (148 residues), 60.5 bits, E=2e-20
• PF12697: Abhydrolase_6
  • amino acids 67 to 325 (259 residues), 35.3 bits, E=2e-12

Best Hits

KEGG orthology group: K00641, homoserine O-acetyltransferase [EC: 2.3.1.31] (inferred from 66% identity to vap:Vapar_5780)

Predicted SEED Role

"Homoserine O-acetyltransferase (EC 2.3.1.31)" in subsystem Methionine Biosynthesis (EC 2.3.1.31)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• L-homocysteine biosynthesis (2/2 steps found)
• L-methionine biosynthesis III (3/4 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (4/6 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.31

Use Curated BLAST to search for 2.3.1.31

Protein Sequence (359 amino acids)

>ABIE41_RS07700 alpha/beta fold hydrolase (Bosea sp. OAE506)
MSSLTGLLRAAVLVASCGLASLAIGADYPAPKRGVWVAKDFRFHTGETLPELKLGYATVG
EPTGEPVVIIHGTAGSAASMLTPIFAGELFGAGQALDASKYFIIIPDAVGAGTSSKPSDG
LRARFPRYHYADMVAAQHRLVSEGLGIRHIRLVLGNSMGGMQTWMWGVSHPDFMDALVPM
ASQPTEMSSRNWMMRRMIIDAVRNDPEWKGGDYTVQPPGFRTANVFFGVATSGGTLAFQH
MAPTREAADKLLDERLKAAFNADANDFLYQWDASRDYNPAPKLEAIKAPVLAINSVDDER
NPPETGIMERELKRLAKARLLLIPASADTRGHGTTGLAKFWAKDLAEWLPTVPRRGGGN