Protein Info for ABIE41_RS03940 in Bosea sp. OAE506

Annotation: folylpolyglutamate synthase/dihydrofolate synthase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01499: bifunctional protein FolC
  • amino acids 23 to 433 (411 residues), 359.6 bits, E=1.4e-111
• PF08245: Mur_ligase_M
  • amino acids 46 to 267 (222 residues), 38.3 bits, E=7.3e-14

Best Hits

KEGG orthology group: K11754, dihydrofolate synthase / folylpolyglutamate synthase [EC: 6.3.2.12 6.3.2.17] (inferred from 69% identity to met:M446_0475)

Predicted SEED Role

"Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17)" (EC 6.3.2.12, EC 6.3.2.17)

MetaCyc Pathways

• folate polyglutamylation (5/5 steps found)
• tetrahydrofolate biosynthesis I (3/3 steps found)
• tetrahydrofolate biosynthesis II (2/3 steps found)
• superpathway of tetrahydrofolate biosynthesis (7/10 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (8/12 steps found)
• superpathway of chorismate metabolism (35/59 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Protein Sequence (440 amino acids)

>ABIE41_RS03940 folylpolyglutamate synthase/dihydrofolate synthase family protein (Bosea sp. OAE506)
MGSSDTVLARLLALHPKLIDLSLGRILTLLERLGDPQKRLPPVIHVAGTNGKGSTIAFMR
AILEAAGLRVHVYTSPHLVRFHERIRLGAPGGGRFVDEAVLVEALERCERANAGDAITFF
EITTAVALLLFAEHEADVVLLEVGLGGRFDATNVIANPACTVVTPVSLDHAEYLGDTVTK
IAGEKAGIFKRGAPAVIAPQEPEPTAVLEATAERIGAARILIGAQDFTVHEAGGRLVYED
GDGLLDLPLPKLAGRHQHVNAGTAIAALRAAGYGGLPSSAFEQGMLNADWPARLQRIARG
KLAELVPGRAELWLDGGHNPDGGRVLAQAMADLADRNPAPLVMIAGLLSTKDAGATLGHF
KGLAQMLFAVPMQNQLAARPAEEVAGLARAAGLTAEVAGSVEQALREIAARDWSAPPRIL
ICGSLYLAGEVLSANGTLPA