Protein Info for ABIE41_RS02475 in Bosea sp. OAE506

Annotation: dihydrolipoamide acetyltransferase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 449 PF00364: Biotin_lipoyl" amino acids 6 to 77 (72 residues), 66 bits, see alignment E=3.4e-22 PF02817: E3_binding" amino acids 163 to 197 (35 residues), 48.9 bits, see alignment 9e-17 PF00198: 2-oxoacid_dh" amino acids 220 to 446 (227 residues), 260.1 bits, see alignment E=2.7e-81

Best Hits

KEGG orthology group: K09699, 2-oxoisovalerate dehydrogenase E2 component (dihydrolipoyl transacylase) [EC: 2.3.1.168] (inferred from 69% identity to mci:Mesci_0883)

Predicted SEED Role

"Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168)" in subsystem Isoleucine degradation or Leucine Degradation and HMG-CoA Metabolism or Valine degradation (EC 2.3.1.168)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.168

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (449 amino acids)

>ABIE41_RS02475 dihydrolipoamide acetyltransferase family protein (Bosea sp. OAE506)
MAERIIKLPDVGEGIAEAELVEWHVKVGDIIREDDLLAAVMTDKATVEIPAPVDGEVTWI
GAEIGDTVAIGSALVKLEVAGEGGTAEPSEPDEKHAVIPRQAEGLSPEPRTDAPESKSAG
ETEPKSAAVAPPQAMTVPRPTKALAAASAPAPALPRRAPGERPVASPAVRLKAREAGIDL
RQLAGTGPAGRILHEDLDAFLLRGPEAERKPGLAEKTGVSEIKVVGLRRKIAEKMALSKA
RIPHITIVEEVDVSALEDLRAVLNKKPTPERPKLTLLPFLMRAMVKALAEQPNLNALYDD
EAGIVRQHAGIHLGIATQTPGGLMVPVVKHAEARDIFGCALELNRIAERARQGTATRDEL
TGSTITITSLGAMGGIATTPVINHPEVAIVGVNKMMIRPVWDGTTFVPRKMMNLSSSFDH
RVVDGWDAAVFVQRLKELLENPATLFIDV