Protein Info for ABIE41_RS02230 in Bosea sp. OAE506
Annotation: amidase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K02433, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A [EC: 6.3.5.6 6.3.5.7] (inferred from 54% identity to azc:AZC_2624)Predicted SEED Role
"Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7)" (EC 6.3.5.6, EC 6.3.5.7)
MetaCyc Pathways
- L-citrulline biosynthesis (7/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- superpathway of L-citrulline metabolism (9/12 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamate and L-glutamine biosynthesis (4/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.5.6, 6.3.5.7
Use Curated BLAST to search for 6.3.5.6 or 6.3.5.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (468 amino acids)
>ABIE41_RS02230 amidase (Bosea sp. OAE506) MVSDPTLLTIREASTLIAERKLSPVELTKACIARTEALDATLSTYVAFTPELALEAAEAA EVEIGTSGPRGPMHGIPVGLKDIYDTAGVATTAHSNLLKSRVPTEDAFTVAKLRESGAVI TGKLGTFEFAIGGPSFDLFTPPARNPWDPARTTGGSSSGSGAAVASGMVLGATGSDTGGS IRSPSSLCGLAGIKPTYGLLSRRGILPLSQSLDHAGPMCWTVEDAALMLQAMAGHDPLDP ACADVAVPDFTEKLDETVKGKTIGVIRHFHETDHQAAPPVLAAIEQAIAVYRSLGCVIKE VTLPPLMDFAAVGLIIMSSEAFTIHEADLRATPELYGEIARDRISMGGLLTGADYVQALR RRQELCAAVAEAMIGVDVLLTAGSPFPAQPIDKVPKWLMYQKPSITMPFNVTGLPALALC AGYEPGTGLPVAMQLVGQPFAETTAFQLGHAYEQATQWRATRPALLAA