Protein Info for ABIE41_RS01220 in Bosea sp. OAE506

Annotation: 1,4-alpha-glucan branching protein GlgB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 732 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 110 to 725 (616 residues), 912.5 bits, see alignment E=6.6e-279 PF02922: CBM_48" amino acids 126 to 207 (82 residues), 80 bits, see alignment E=2e-26 PF00128: Alpha-amylase" amino acids 269 to 348 (80 residues), 30.8 bits, see alignment E=3.6e-11 PF02806: Alpha-amylase_C" amino acids 631 to 728 (98 residues), 77.2 bits, see alignment E=1.5e-25

Best Hits

Swiss-Prot: 67% identical to GLGB1_XANC8: 1,4-alpha-glucan branching enzyme GlgB 1 (glgB1) from Xanthomonas campestris pv. campestris (strain 8004)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 66% identity to xop:PXO_03669)

MetaCyc: 53% identical to 1,4-alpha-glucan branching enzyme (Mycobacterium tuberculosis H37Rv)
1,4-alpha-glucan branching enzyme. [EC: 2.4.1.18]

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.18

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (732 amino acids)

>ABIE41_RS01220 1,4-alpha-glucan branching protein GlgB (Bosea sp. OAE506)
MTEAAVERVQPLPMETATALAQGRWGVPSAILGPHQGPAGRYLRVFVPGAEAVTVRQDGR
DDVRLRPAEPEGLFVGDLADGLYRLAIRWPGGEQLTEDPYAFGPLLGDLDLHLIGEGRHL
ELARALGANACSAEGVPGVRFAVWAPNARRVSVVGTFNSWDGRRHPMNRRGGGGVWELFI
PRLSSGELYKYEIVDRDGHLLPQKADPLARAAELPPGTASIVPRPPSHVWGDQAWMAARA
ERQAADAPISIYELHPGSWVRGPNGDMLDWRNLADRLVPYVKAMGFTHIELMPVAEHPFT
GSWGYQPLGLFAPTRRFGEPEDFALFVEACHREEIGVIVDWVPAHFPADAHGLARFDGTA
LYEHEDPREGFHKDWNTLIYNFGRREVSNFLIASALHWLEYFHVDGLRVDAVASMLYRDY
SRQPGEWVPNAQGGRENLEAVAFLRELNTLVAQRQPGAIMIAEESTAWPGVTRPTAEGGL
GFAYKWNMGWMHDTLHYLQRDPLYRRHHHDEMTFGLVYAFSEQFMLPLSHDEVVHGKGSL
LEKMPGDLWQKFAGLRAYFAFMWTHPGKKLLFMGGEIAQRREWNHDGEIDWDLLEDPLHR
GMQALVGDLNRLYAAWPALHASDGEPSGFRWLVQDDRENSVFAFLRQAPGEKPVVTVVNL
TPVTRWNYRIGVPRAGGWREVFNSDAGAYGGSGVGNGGWVEARAESLGADPAFVEIALPP
LGALVFVPDGDA