Protein Info for ABIE41_RS00990 in Bosea sp. OAE506

Annotation: NAD-dependent epimerase/dehydratase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 685 PF02719: Polysacc_synt_2" amino acids 331 to 455 (125 residues), 26.7 bits, see alignment E=9.6e-10 PF04321: RmlD_sub_bind" amino acids 331 to 557 (227 residues), 31 bits, see alignment E=4.4e-11 PF01370: Epimerase" amino acids 331 to 592 (262 residues), 162.3 bits, see alignment E=4.1e-51 PF01073: 3Beta_HSD" amino acids 332 to 530 (199 residues), 53.8 bits, see alignment E=4.7e-18 PF16363: GDP_Man_Dehyd" amino acids 333 to 656 (324 residues), 132.7 bits, see alignment E=6.7e-42 PF07993: NAD_binding_4" amino acids 397 to 542 (146 residues), 31.2 bits, see alignment E=3.8e-11

Best Hits

KEGG orthology group: K12454, CDP-paratose 2-epimerase [EC: 5.1.3.10] (inferred from 62% identity to rlg:Rleg_5580)

Predicted SEED Role

"Nucleoside-diphosphate-sugar epimerases"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.3.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (685 amino acids)

>ABIE41_RS00990 NAD-dependent epimerase/dehydratase family protein (Bosea sp. OAE506)
MSGIASATAGQPAVGFVEWFRPGEHRRVEAAIAGLQAAGVNRLRSHLSWAEYHGDGGRDW
YDWLIPRLGQSFDFLPCIHYTPPSLSRTGASSGAPRVLKDYADFIDGVLTRYGEHFRDVE
VWNEPNNLLDWDWRVDPDWSLFCEMIGNAAYWVQHRGWRAVLGGPSPFDPHWLELMGERG
VLGVVSAVGLHGFPGTWDSEVGTWRGYDAHIAEARAILARHNPDCEIWITEAGYSTWRED
EAEQVRRFTEAREAPADRLYWYGWQDVPRDVPVQEGLYFDPRHYHLGVTREDGRPKLLRR
LLETEGSAGFDTIMPDAPPARPLRAVRPAPVVVFGGAGFIGTNLCERLLGEGQRVMLFDS
LGRPGVERNLRWLQERHGAALETRLSDIRDEAAVRSAVTGAKACFHLAAQVAVTDSLVDP
RHDFAVNAQGTLTILEALRLQAPETPLVFASTNKVYGSLASLDMEQTENGWAPANGLFRR
HGVNETRPLDFCTPYGCSKGTADQYVLDYAHSFGLRTAVLRMSCIFGPRQFGTEDQGWVA
HFLLKALRGEPITIFGDGQQVRDILHVADAVDAYCRVLGAIDSVSGRAFNLGGGPANSVS
LATVIAEIGMLIGRPPTLHHQDWRAGDQPWFVADTRALQKTLGWRASFDWRDGLRDLHGW
ATREFGHGPVAPVAAAASARRRVSA