Protein Info for ABIE40_RS27135 in Rhizobium sp. OAE497

Annotation: CHAD domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 484 PF01928: CYTH" amino acids 4 to 170 (167 residues), 73.8 bits, see alignment E=1.6e-24 PF05235: CHAD" amino acids 220 to 441 (222 residues), 187.7 bits, see alignment E=3.4e-59

Best Hits

KEGG orthology group: None (inferred from 45% identity to rhi:NGR_b14510)

Predicted SEED Role

"Adenylate cyclase (EC 4.6.1.1)" in subsystem cAMP signaling in bacteria (EC 4.6.1.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.6.1.1

Use Curated BLAST to search for 4.6.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (484 amino acids)

>ABIE40_RS27135 CHAD domain-containing protein (Rhizobium sp. OAE497)
MSSETEIKLDLSQDACVALLGSDLLGEPDRTLQQRSTYFDTPERTLWKQGYSLRIRKVGD
ARTQTVKATGPSRALFARSEWEIPVEGDEPVLDHTSPLIGEFGKELRLEPVFDVVVERRL
WNVDENGSRIEVVGDQGEVISGERRSPIRELEIELKDGSPGDLFALARKVDAIAAFRFGV
ISKSEGGVLLIDAQKTVFKSEPIYLDRGGSPAFAFQSVALSCLRHFRLNEDVFMRGGNPE
ALHQARVALRRLRSAFSLFKPILENQEPQWLKDELRWLAGVLGEARNVDVLLAKAVDPDF
IWRLKTARKATYRDALDALESSRTRALMLDVVEWLQCGDYLSGGLGTGEASDASQAFAAG
VLEKQRKRLKKDARALKKLDDQDRHEIRKDAKKFRYAAEFFASLFDDKRSARRHSKFLKA
MEALQDQLGALNDLVTEPDVLNRHGLSNHPAADTVMYHGAKGKLIDAAQDALDEVLDTKR
FWRL