Protein Info for ABIE40_RS26485 in Rhizobium sp. OAE497

Annotation: mandelate racemase/muconate lactonizing enzyme family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02746: MR_MLE_N
  • amino acids 41 to 125 (85 residues), 39 bits, E=8.6e-14
• PF13378: MR_MLE_C
  • amino acids 233 to 417 (185 residues), 154 bits, E=4.9e-49

Best Hits

KEGG orthology group: None (inferred from 91% identity to ret:RHE_PE00103)

Predicted SEED Role

"Gluconate dehydratase (EC 4.2.1.39)" in subsystem Entner-Doudoroff Pathway (EC 4.2.1.39)

MetaCyc Pathways

• Entner-Doudoroff pathway III (semi-phosphorylative) (7/9 steps found)
• Entner-Doudoroff pathway II (non-phosphorylative) (5/9 steps found)

KEGG Metabolic Maps

• Pentose phosphate pathway

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.39

Use Curated BLAST to search for 4.2.1.39

Protein Sequence (453 amino acids)

>ABIE40_RS26485 mandelate racemase/muconate lactonizing enzyme family protein (Rhizobium sp. OAE497)
MKEADGAEDALDRVNQNSKPSDLRITDIRVAEIVGAPFTSALLKIYTNQGIVGLGEVRDG
ASATYALMLKSRLLGENPCDIDRLFRRIKQFGGHGRQGGGVSAVEIALWDLAGKAYGVPV
YQMLGGRFREKVRVYCDTDATVPSGTETGKRLKERMDLGFTFLKMDLGLMQIADVPGAVV
FPAGSLEGYRDSPTRGPLKTADERRLRNAAYDLHNVQHPFTGLHFTDKGLDLLEQYIAEV
REVTGMNVPLAIDHIGHISMQNGIRLARRIEKYVPAWLEDVIPWQYAQQYRQLQDATTVP
ICTGEDIYLKEGFQPLLDSGGVSVIHPDLLTSGGILETKKIGDMAQDRGVAMAIHMAESP
IAAMAAAHVATATENFMALEYHSADVDWWDDIVTGLPRPLVKDGFITVPDKPGLGIDDVV
DEVILQHLQPGVAGIWQPTDHWDDEYSWDRTWS