Protein Info for ABIE40_RS26425 in Rhizobium sp. OAE497

Annotation: formaldehyde dehydrogenase, glutathione-independent

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 396 TIGR02819: formaldehyde dehydrogenase, glutathione-independent" amino acids 3 to 391 (389 residues), 720.9 bits, see alignment E=1.5e-221 PF08240: ADH_N" amino acids 35 to 144 (110 residues), 82.2 bits, see alignment E=2.3e-27 PF01262: AlaDh_PNT_C" amino acids 186 to 242 (57 residues), 23.3 bits, see alignment E=4e-09

Best Hits

Swiss-Prot: 73% identical to FADH_PSEAE: Glutathione-independent formaldehyde dehydrogenase (fdhA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00148, glutathione-independent formaldehyde dehydrogenase [EC: 1.2.1.46] (inferred from 94% identity to atu:Atu1567)

Predicted SEED Role

"Threonine dehydrogenase and related Zn-dependent dehydrogenases" in subsystem Threonine degradation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.46

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (396 amino acids)

>ABIE40_RS26425 formaldehyde dehydrogenase, glutathione-independent (Rhizobium sp. OAE497)
MSKNRGVVYLGPGKVEVRDIDDPKLEAPDGRRIEHGVILKVISTNICGSDQHMVRGRTTA
MPGLVLGHEITGEVIEKGVDVEMLEIGDIVSVPFNVACGRCRCCKSQDTGVCLTVNPSRA
GGAYGYVDMGGWIGGQARYVTIPYADFNLLKIPDRDKAMAKIRDLTMLSDILPTGFHGAV
RAGVGVGSTVYVAGAGPVGLAAAASARILGAAVVMIGDFNKDRLAHAAKVGFEPIDLSKS
DRLGDMIAQVVGTNEVDSAIDAVGFEARGHSGGEQPAIVLNQMMEITRAAGSIGIPGLYV
TEDPGAVDAAAKQGSLSLRFGLGWAKAQSFHTGQTPVLKYNRQLMQAILHDRLPIADIVN
AKVISLEDAAQGYESFDQGAATKFVLDPHGVVSKAA