Protein Info for ABIE40_RS26380 in Rhizobium sp. OAE497
Annotation: malto-oligosyltrehalose trehalohydrolase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 56% identity to sme:SM_b21447)Predicted SEED Role
"Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141)" in subsystem Trehalose Biosynthesis (EC 3.2.1.141)
MetaCyc Pathways
- glycogen biosynthesis I (from ADP-D-Glucose) (3/4 steps found)
- glycogen biosynthesis II (from UDP-D-Glucose) (3/4 steps found)
- trehalose biosynthesis V (2/3 steps found)
- glycogen biosynthesis III (from α-maltose 1-phosphate) (4/8 steps found)
- starch biosynthesis (5/10 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.4.1.18, 3.2.1.141
Use Curated BLAST to search for 2.4.1.18 or 3.2.1.141
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (591 amino acids)
>ABIE40_RS26380 malto-oligosyltrehalose trehalohydrolase (Rhizobium sp. OAE497) MQKTGKRRMAWGPQAIDGGMRFRLWAPGEAEVSLRLNGRDMPMVRFEDGWFEAAIADVRA GDAYAFVTGDGRAVPDPAARRQRGDVHGLSLVASSDYRWRSDSWTGRDWEEAVISEIHIG TFTQAGTLAGAAEHLADLAAAGITAIEIMPVAQFAGQRGWGYDGVLHYAPHPAYGTPDEF RAFIDAAHAEGIMVLLDVVYNHFGPEGNYLHSYAPEFFRTDRATPWGDAIDFSQPAVRRY FIDNALYWIGDFRLDGLRLDAVEQIHDTSARHVLDELAQEVRAAFADRKVHLVIEDQRNL VSLLERDTRGGVKAFTAEWNDDFHHVAHIIATGEADGHYTAFAGELWQKTRLALQHGFVY PDRADPPVLPDGERVYLPPEAFINFLQNHDQIGNRAFGERLVSLAPQETVEALTAIMMLS PQIPFLFMGEDYGESQPFYFFCDYTGELGEIVRKGRMAEAEGFGGLKDGRSKDELPDPNA RSTFDGSKLQWQRAESPEGRRRRDLVRELARLRQLHIVPLIKETGRVYAKALETTDGGLA IDWHMEGAVLQLRANLSGEPLTLPATSGRSIYGAPPDSGLYAQNTVVFAIA