Protein Info for ABIE40_RS25060 in Rhizobium sp. OAE497

Annotation: FAD-dependent monooxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01494: FAD_binding_3
  • amino acids 6 to 370 (365 residues), 371.6 bits, E=4.5e-115
• PF21274: Rng_hyd_C
  • amino acids 465 to 584 (120 residues), 72 bits, E=4.6e-24

Best Hits

Swiss-Prot: 56% identical to TFDB_CUPNJ: 2,4-dichlorophenol 6-monooxygenase (tfdB) from Cupriavidus necator (strain JMP 134 / LMG 1197)

KEGG orthology group: K10676, 2,4-dichlorophenol 6-monooxygenase [EC: 1.14.13.20] (inferred from 81% identity to rlg:Rleg_5534)

MetaCyc: 56% identical to 2,4-dichlorophenol 6-monooxygenase I (Cupriavidus pinatubonensis JMP134)
Phenol 2-monooxygenase. [EC: 1.14.13.7]; 2,4-dichlorophenol 6-monooxygenase. [EC: 1.14.13.7, 1.14.13.20]; 1.14.13.20 [EC: 1.14.13.7, 1.14.13.20]

Predicted SEED Role

"FAD-binding monooxygenase, PheA/TfdB family, similarity to 2,4-dichlorophenol 6-monooxygenase"

MetaCyc Pathways

• 2,4-dichlorophenoxyacetate degradation (1/2 steps found)
• 4-chloro-2-methylphenoxyacetate degradation (1/2 steps found)
• chlorinated phenols degradation (1/4 steps found)

KEGG Metabolic Maps

• 1,4-Dichlorobenzene degradation
• Naphthalene and anthracene degradation
• Toluene and xylene degradation
• gamma-Hexachlorocyclohexane degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.13.20 or 1.14.13.7

Protein Sequence (584 amino acids)

>ABIE40_RS25060 FAD-dependent monooxygenase (Rhizobium sp. OAE497)
MADITTDVLIIGTGPAGSATAALLATYGVEPLVINRYRWLATTPRAHITNQRTMEVLRDL
GRDVEDEAYLFATEQELMGQNVFCTAVAGEEIGRMQSWGNHPLSQAEHILTSPGRMNDLP
QTYMEPLLFKTACSRGAQSRMSTEYLSHVQDGEGVTTTCLDRLTGKELTIRSKFLIGADG
GNSKVAEHAGLTFEGKMGVAGSMNILFEADLSRYVAHRPSVLYWVLQPGADVGGIGMGLV
RMVRPWNEWLIVWGYDINQPAPQVDDAFATKVVRELVGVPDLAPKIKSVSTWTVNNMYAT
TLSKGRVFCMGDAVHRHPPSNGLGSNTSIQDGFNLAWKLAMVLKGQAGAGLLDSYQAERA
PVAKQIVTRANKSIEEFGPIFKSLGLLDSVDPVKMQQNMDARCNDTDDAERQRAAIREAI
ASKVYEFDAHGVEMNHRYASNAVVKDDQPEPKFEKDAELHFQPTSWPGARLPHVWLFGQN
GEKISSLDLTGHGVFTILTGIGGDGWQAAAKAVAKEFGIQINVHKIGPRQVWQDLTGDWA
RAREIRDSGALLVRPDHHVAWRAEAAVPDAEGELRRVLKTVLDR