Protein Info for ABIE40_RS25055 in Rhizobium sp. OAE497

Annotation: intradiol ring-cleavage dioxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04444: Dioxygenase_N
  • amino acids 28 to 100 (73 residues), 101.4 bits, E=1.9e-33
• PF00775: Dioxygenase_C
  • amino acids 126 to 290 (165 residues), 155.2 bits, E=1.4e-49

Best Hits

KEGG orthology group: K03381, catechol 1,2-dioxygenase [EC: 1.13.11.1] (inferred from 85% identity to rlg:Rleg_5533)

MetaCyc: 74% identical to hydroxyquinol 1,2-dioxygenase (Rhizobium sp. MTP-10005)
Hydroxyquinol 1,2-dioxygenase. [EC: 1.13.11.37]

Predicted SEED Role

"Catechol 1,2-dioxygenase (EC 1.13.11.1)" in subsystem Catechol branch of beta-ketoadipate pathway (EC 1.13.11.1)

MetaCyc Pathways

• aromatic compounds degradation via β-ketoadipate (8/9 steps found)
• superpathway of salicylate degradation (6/7 steps found)
• catechol degradation III (ortho-cleavage pathway) (5/6 steps found)
• catechol degradation to β-ketoadipate (3/4 steps found)
• resorcinol degradation (2/3 steps found)
• γ-resorcylate degradation I (2/4 steps found)
• γ-resorcylate degradation II (2/4 steps found)
• 4-aminophenol degradation (2/4 steps found)
• 2,4,5-trichlorophenoxyacetate degradation (5/9 steps found)
• 4-nitrophenol degradation II (2/5 steps found)
• mandelate degradation to acetyl-CoA (7/18 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (12/35 steps found)

KEGG Metabolic Maps

• 1,4-Dichlorobenzene degradation
• Benzoate degradation via hydroxylation
• Carbazole degradation
• Fluorobenzoate degradation
• Toluene and xylene degradation
• gamma-Hexachlorocyclohexane degradation

Isozymes

Compare fitness of predicted isozymes for: 1.13.11.1

Use Curated BLAST to search for 1.13.11.1 or 1.13.11.37

Protein Sequence (295 amino acids)

>ABIE40_RS25055 intradiol ring-cleavage dioxygenase (Rhizobium sp. OAE497)
MSSDEKGYFTEASSVEVVTSRNVNAKCERLKQVMEVVTRKLHEAVKELEPTQDEWMEAIM
FLTRTGHTCTDWRQEFILLSDVLGVSMLVDAINNRKPSGASESTVLGPFHVADAPELPMG
ANICLDQKGEDMVISGRILDTDGKPIENAVIDVWQANDEGFYDVQQKGIQPDFNLRGIFR
TGADGHYWFRAVKPKFYPIPDDGPVGQLLAQLGRHPYRPAHLHYIIKADGFETLTTHIFD
PDDPYIHSDAVFGVKESLLAEFRLKEDAGRARVLEFADKFWDVEHDFVLARDSGS