Protein Info for ABIE40_RS21585 in Rhizobium sp. OAE497
Annotation: GMC family oxidoreductase N-terminal domain-containing protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 55% identical to SDH_GLUOY: L-sorbose 1-dehydrogenase from Gluconobacter oxydans
KEGG orthology group: K00119, [EC: 1.1.99.-] (inferred from 59% identity to bpy:Bphyt_4183)MetaCyc: 55% identical to L-sorbose dehydrogenase, FAD dependent (Gluconobacter oxydans UV10)
L-sorbose 1-dehydrogenase. [EC: 1.1.99.32]
Predicted SEED Role
"Choline dehydrogenase (EC 1.1.99.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.1.99.1)
MetaCyc Pathways
- choline-O-sulfate degradation (3/3 steps found)
- choline degradation I (2/2 steps found)
- glycine betaine biosynthesis I (Gram-negative bacteria) (2/2 steps found)
- glycine betaine biosynthesis II (Gram-positive bacteria) (2/2 steps found)
- 2-keto-L-gulonate biosynthesis (3/4 steps found)
- L-ascorbate biosynthesis III (D-sorbitol pathway) (2/4 steps found)
KEGG Metabolic Maps
- Aminophosphonate metabolism
- Benzoate degradation via hydroxylation
- Glycine, serine and threonine metabolism
- Limonene and pinene degradation
Isozymes
Compare fitness of predicted isozymes for: 1.1.99.-, 1.1.99.1
Use Curated BLAST to search for 1.1.99.- or 1.1.99.1 or 1.1.99.32
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (536 amino acids)
>ABIE40_RS21585 GMC family oxidoreductase N-terminal domain-containing protein (Rhizobium sp. OAE497) MPDYIIVGGGSAGCVLAARLSEDPDVSVTLLEAGPRDSNMYIHLPVGFFKMTAGPLIWGY ETVAGREIAGRKMVYPQARVLGGGSSINAQVFTRGCPEDYDGWRDLEGCAGWGYEDVRPY FRKSEGNDTFGGAHHGTEGPLGVSSQNPHPLTRVFVRAAQEAGLPFNPDFNAGSQAGAGF YQTTTKNGRRSSTAVGYLKPAVHRKNLTVRTDTAVNRIVIENGCAVGIEIIENGRPVLLR AEREVILTSGAIGSPKMLMLSGLGPADELRALDIDVVRDLPQVGKNLQDHMDVDVLAELS GSHGIDRYKKRHWQVAAGIEYALFGKGPVASNIVEGGGFWWGDKAEKTPDLQFHFLPGAG VEEGIGGVPGGNGCTLNSYHVRPRSRGTVTLRSKDPSVPPAIDPNAFAEPYDLERAVDGI KICQEILSQPAFRPFVRRLHLPDGNIRTQEEYRQFARQYGRSAYHPVGTCRMGGGEDAVV DPDLKVRGIDRLRVCDSSVMPRLISSNTNAATIMIAEKAADILKGVLQRQPQHDYA