Protein Info for ABIE40_RS20025 in Rhizobium sp. OAE497

Annotation: alpha-hydroxy acid oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01070: FMN_dh
  • amino acids 16 to 378 (363 residues), 430.7 bits, E=2.2e-133

Best Hits

KEGG orthology group: K00101, L-lactate dehydrogenase (cytochrome) [EC: 1.1.2.3] (inferred from 85% identity to axy:AXYL_03805)

Predicted SEED Role

"L-lactate dehydrogenase (EC 1.1.2.3)" in subsystem L-rhamnose utilization or Lactate utilization or Respiratory dehydrogenases 1 (EC 1.1.2.3)

MetaCyc Pathways

• methylglyoxal degradation V (2/3 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (1/8 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.3

Use Curated BLAST to search for 1.1.2.3

Protein Sequence (381 amino acids)

>ABIE40_RS20025 alpha-hydroxy acid oxidase (Rhizobium sp. OAE497)
MRLTDCHNFHDFRRLAKRRLPGPIFNYIDGAADDEVTYRRNTFSFESCDLLPSVLRGVSN
VDMSVTVMGQRLAMPVYCSPTALQRLFHHEGERAVARAAGKFGTMFGVSSLGTVSLEEAR
RISGAPQVYQFYFHKDRGLNREMMARAKEAGVQVMMLTVDSITGGNRERDKRTGFSIPFR
LNLAGIAQFAVKPGWALNFFTHEKFKLPQLDSHVDMGSGTMSISRYFTEMLDPAMNWDDV
AEMVKTWGGQFCLKGIMTVEDAKRAIDIGCTGIVLSNHGGRQLDGSRSGFDQLAEIVDAV
GDKIDVMIDGGVQRGTHVLKALSLGAKAVGLGRYYLFPLAGAGQAGVERALTLMQAELER
GMKLMGCSSVDQLTRANVRFR