Protein Info for ABIE40_RS19515 in Rhizobium sp. OAE497

Annotation: ATP-dependent helicase HrpB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 820 TIGR01970: ATP-dependent helicase HrpB" amino acids 9 to 819 (811 residues), 999.2 bits, see alignment E=9e-305 PF00270: DEAD" amino acids 21 to 170 (150 residues), 46.7 bits, see alignment E=6.3e-16 PF00271: Helicase_C" amino acids 211 to 333 (123 residues), 50.4 bits, see alignment E=5e-17 PF08482: HrpB_C" amino acids 675 to 806 (132 residues), 153.6 bits, see alignment E=8.2e-49

Best Hits

KEGG orthology group: K03579, ATP-dependent helicase HrpB [EC: 3.6.4.13] (inferred from 84% identity to rle:RL0059)

Predicted SEED Role

"macromolecule metabolism; macromolecule synthesis, modification; dna - replication, repair, restr./modif."

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (820 amino acids)

>ABIE40_RS19515 ATP-dependent helicase HrpB (Rhizobium sp. OAE497)
MDIASLPVLPVSHVLSDIGAALESQRRAVLSAPPGAGKTTLVPLFLLAQSWRGDGKIILL
EPRRLAARAAASRMASLLGESVGQTVGYRMRLDNRVSAATRIEVVTEGVFARMILDDPEL
SGVSTVIFDEFHERSLDADFGLALSIDVQGALRDDLRILVMSATLDVERVATLLDNPPII
ESMGRGFPIDIRYQDRPGGERIEDTVTRAILDYHATETGSILAFLPGQAEILRTTERLEG
RLGASTLIAPLYGNLSQKEQDAAIRPAAAGTRKVVLATSIAETSITIDGVRMVIDSGLQR
LPVFEPSTGITRLETVRVSRASADQRAGRAGRTEPGIALRLWHQGQTAALPAFTPPQILS
SDLSGLVLDLAHWGVQDPKTLAFVDQPPAATLAEARSLLIDLGALAADGSLTERGRTMRE
MALPPRLAAMVVSAGEHGDAREAALLAVLMTEQGLGGASIDLEERLRRFKTEKGERAESA
RRLAQRLVSGLDKVKSTASPTAGQLLIHAFPDRIALQRGARGRYVMANGRGGELAETERL
AGSQMLVIADLTGRAAQARILAAAEITRGDVEAELPGAIKTGEQTYFDLPSRQVRARKAT
RLGAIVFEETPLPRPSGAATAKALADGIRQLGLATLPFSKEAAQLRERIGFLYRTIGEPW
PDMSDEALLERLDDWFVPFQADVRGIADISAGSLSNGLISLVPHALQRDLAKLAPTHFEA
PTGQRHPIQYDGEEPLLTIRVQELFGLKDHPAIGGGRLPLLLELTSPAHRPIQTTRDLPG
FWAGSWKDVRAEMRGRYPRHPWPERPEEALPTTRAKPRGT