Protein Info for ABIE40_RS19220 in Rhizobium sp. OAE497

Annotation: tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details PF10396: TrmE_N" amino acids 5 to 119 (115 residues), 115.1 bits, see alignment E=3.3e-37 TIGR00450: tRNA modification GTPase TrmE" amino acids 16 to 434 (419 residues), 262.9 bits, see alignment E=6.4e-82 PF12631: MnmE_helical" amino acids 122 to 431 (310 residues), 163.8 bits, see alignment E=9.9e-52 TIGR00231: small GTP-binding protein domain" amino acids 217 to 311 (95 residues), 59.9 bits, see alignment E=2.6e-20 PF01926: MMR_HSR1" amino acids 218 to 311 (94 residues), 76 bits, see alignment E=3.9e-25

Best Hits

Swiss-Prot: 68% identical to MNME_RHIL3: tRNA modification GTPase MnmE (mnmE) from Rhizobium leguminosarum bv. viciae (strain 3841)

KEGG orthology group: K03650, tRNA modification GTPase (inferred from 68% identity to rlg:Rleg_4257)

Predicted SEED Role

"GTPase and tRNA-U34 5-formylation enzyme TrmE" in subsystem Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (434 amino acids)

>ABIE40_RS19220 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE (Rhizobium sp. OAE497)
MTRQTIYALSSGGLPSGVAVVRISGPATAAALQHLVGTLPSPRQASLRTIRNPNGDPIDS
GLILFFPSPASFTGEDCAELQIHGGRAVVQSLLQCLSEIPGLRLAVEGEFSRRAFENGKL
DLVEIEGLADLIAAETEMQRRLAVEHSSGHLSKLYDSWAERLTRCRALIEAELDFADEDD
VPGSVSDQVWAEVNRLQSEISAHLANADAGEIIRDGFKVVIAGAPNAGKSSLMNALAQRD
VAIVTDIAGTTRDVLQVDLDIGGFLVKLYDTAGLRETEERVEREGIRRAENLVAQADLVL
YLHDQSSGLSAEHAVSGDARVNIRTKADLAVASDAFDLSISVQTGEGLDELRLLIGDRIA
RTVRPESLALPSRLRHKDSLAECLRAIGIALSAGELDLRAEELRVAANALGRITGRVDVE
NLLDVIFSEFCIGK