Protein Info for ABIE40_RS16520 in Rhizobium sp. OAE497

Annotation: sarcosine oxidase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 997 TIGR01372: sarcosine oxidase, alpha subunit family" amino acids 4 to 996 (993 residues), 1575.6 bits, see alignment E=0 PF13510: Fer2_4" amino acids 18 to 102 (85 residues), 93.9 bits, see alignment E=2.5e-30 PF12831: FAD_oxidored" amino acids 172 to 219 (48 residues), 36.2 bits, see alignment 2.3e-12 PF07992: Pyr_redox_2" amino acids 172 to 429 (258 residues), 48.4 bits, see alignment E=4.7e-16 PF01266: DAO" amino acids 172 to 260 (89 residues), 36 bits, see alignment E=3e-12 PF00890: FAD_binding_2" amino acids 172 to 214 (43 residues), 29.3 bits, see alignment (E = 2.7e-10) PF13450: NAD_binding_8" amino acids 175 to 210 (36 residues), 22.6 bits, see alignment (E = 5.1e-08) PF17806: SO_alpha_A3" amino acids 515 to 598 (84 residues), 105.5 bits, see alignment E=7.6e-34 PF01571: GCV_T" amino acids 613 to 879 (267 residues), 234.5 bits, see alignment E=6.3e-73 PF08669: GCV_T_C" amino acids 904 to 989 (86 residues), 68.6 bits, see alignment E=1.8e-22

Best Hits

KEGG orthology group: K00302, sarcosine oxidase, subunit alpha [EC: 1.5.3.1] (inferred from 88% identity to ara:Arad_3878)

Predicted SEED Role

"Sarcosine oxidase alpha subunit (EC 1.5.3.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.5.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.3.1

Use Curated BLAST to search for 1.5.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (997 amino acids)

>ABIE40_RS16520 sarcosine oxidase subunit alpha (Rhizobium sp. OAE497)
MSGANRIAGKGRLTPAKTARFTFDGKSYTALEGDTVASALIANGIHLVGRSFKYHRPRGI
LSAGAEEPNALIDVARDSARKQPNVRATVQEIFDGMIVKSQNRFPSLAFDIGGVNNLMSP
FFAAGFYYKTFMWPKAAWKHIYEPMIRRAAGLGVAPTEADPDHYASRYAHCDVLVAGAGV
AGLSAALAAAEAGASVIIVDEQADVGGALRYDSGVKIDGVDGYTWAQQTAAKLRAMDNVQ
VLTRTTAFGYYNHNFIALAERVTDHMAKPGRDLPRERLWQVRAKRVILANGSIERHMVFA
NNDRPGIMLASAARMYLNQYGVAVGAKVGVYTAHDSAYEAAFDLKRAGVAIAAIVDCRPQ
PGAAVLAEARSLGIEVLTGHAVIDTAGKLRVSSMSVVRNGGGSPRKIAIDALLVSAGWTP
SVHLFSQSRGKVKFEPETERFLPGTYAQECLSVGACNGTDDLQQTIEESLAAGELMAQAS
GKQASGKISISAEQAFQWTGGMIGAAEGAGPETSAKAFIDFQHDVCAKDIRLAVREGMHS
IEHIKRFTTNGMASDQGKLSNMHGLAIAAEMLGKDIPQVGLTTFRAPYTPVTYGTLIAHS
RGELFDPTRKTPLHQWEEAQGAVFEDVGNWKRAWFYPRAGETMHQAVARECKTARDVAGV
FDASTLGKIEVVGPDAAQFMNLMYTNAWDTLKPGKCRYGIMTREDGFVYDDGVVGRLSED
RFHVTTTTGGAPRVLNHMEDYLQTEFPHLNVWLTSVTEQWAVIAVQGPKAREIIAPLVEG
VDISNEAFPHMSVAECKVMGVPARLFRVSFTGEAGFEINVPADYGQAVLEAVWANAEPLG
ACAYGTETMHVLRAEKGYIIVGQDTDGTVTPDDAGLSWAVSKKKTDFVGIRGLKRIDLTK
EGRKQLVGLVTKDPKVVLEEGAQVVANPNEPKPMTMLGHVTSSYWSENCGRSIAFALVAG
GRARMGETLYIPMPDKTIAVEVTDMVFFDKEGGRING