Protein Info for ABIE40_RS15550 in Rhizobium sp. OAE497

Annotation: heavy metal translocating P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 737 transmembrane" amino acids 128 to 145 (18 residues), see Phobius details amino acids 151 to 168 (18 residues), see Phobius details amino acids 175 to 197 (23 residues), see Phobius details amino acids 203 to 222 (20 residues), see Phobius details amino acids 353 to 373 (21 residues), see Phobius details amino acids 384 to 408 (25 residues), see Phobius details amino acids 686 to 706 (21 residues), see Phobius details amino acids 712 to 733 (22 residues), see Phobius details PF00403: HMA" amino acids 9 to 67 (59 residues), 56.4 bits, see alignment 6.5e-19 TIGR01525: heavy metal translocating P-type ATPase" amino acids 183 to 732 (550 residues), 589.1 bits, see alignment E=2.2e-180 TIGR01512: cadmium-translocating P-type ATPase" amino acids 183 to 542 (360 residues), 320.7 bits, see alignment E=3.4e-99 TIGR01511: copper-translocating P-type ATPase" amino acids 202 to 732 (531 residues), 411.3 bits, see alignment E=1.5e-126 TIGR01494: HAD ATPase, P-type, family IC" amino acids 206 to 708 (503 residues), 271.2 bits, see alignment E=2.7e-84 PF00122: E1-E2_ATPase" amino acids 234 to 414 (181 residues), 185.4 bits, see alignment E=1.5e-58 PF00702: Hydrolase" amino acids 430 to 644 (215 residues), 121 bits, see alignment E=1.7e-38

Best Hits

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 78% identity to rec:RHECIAT_CH0003992)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (737 amino acids)

>ABIE40_RS15550 heavy metal translocating P-type ATPase (Rhizobium sp. OAE497)
MAETSQAKYRVGGMDCASCASKIDTAVRRLPGVEDVAVSVTAGTMTVSHDGSSDLGAIEK
RVTGLGYSVVPMPLGKQSAPAPSSHAHHDHDHAGHDHSDHDHDHAEIEGLHGHDHAPMDG
PWWKSRKGQLTILSGIAFAVAYAAGHLLPQISAYAFTVAMLIGLIPIARRAVMAALAGTP
FSIEMLMTIAAVGAVIINAAEEAAAVVFLFLVGELLEGVAAGRARASIQSLTTLVPKSAL
LEENGTTREVPAEALAIGSIILVRPGDRISADGTIVSGESAIDESPVTGESVPVRKSPDD
TVFAGTVNGDTALRIRVTAAAADNTIARVVKLVEEAQESKAPTERFIDRFSRYYTPGVVA
VAALVAILPPLIAGGSWSEWVYKGLAILLIGCPCALVISTPAAIAASLSSGARRGLLLKG
GAVLEGLGKITAVALDKTGTLTEGKPKVTDIVAFGSFTEAQVLSRAAVLERSSSHPLALA
ILNRAAADKVPVPPAFEVAAIAGKGMTGRVGGEQLDLFSPAAVREQLSPEQDSAIQALND
QGKTVSILLVNGAIAGVIAMRDEPRDDAAAGLKALTARGVKVVMLTGDNVRTAKAIGEQL
GIEVRAELMPEDKQRIVSELKREGFTVAKVGDGINDAPALAAADIGIAMGGGTDVALETA
DAAILHGRVGDVASMIALSQRTMRNIFQNITIALGLKAVFLVTTISGITGLWPAILADTG
ATVLVTINALRLLRPAR