Protein Info for ABIE40_RS14535 in Rhizobium sp. OAE497

Annotation: dihydropyrimidinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02033: dihydropyrimidinase
  • amino acids 4 to 452 (449 residues), 592.2 bits, E=3.6e-182
• PF01979: Amidohydro_1
  • amino acids 47 to 419 (373 residues), 79.7 bits, E=2.5e-26

Best Hits

Swiss-Prot: 51% identical to HYDA_PSEAE: D-hydantoinase/dihydropyrimidinase (dht) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01464, dihydropyrimidinase [EC: 3.5.2.2] (inferred from 89% identity to ara:Arad_3447)

MetaCyc: 48% identical to dihydropyrimidinase (Arabidopsis thaliana col)
Dihydropyrimidinase. [EC: 3.5.2.2]

Predicted SEED Role

"Dihydropyrimidinase (EC 3.5.2.2)" in subsystem Hydantoin metabolism or Pyrimidine utilization (EC 3.5.2.2)

MetaCyc Pathways

• superpathway of pyrimidine ribonucleosides degradation (5/5 steps found)
• thymine degradation (3/3 steps found)
• uracil degradation I (reductive) (3/3 steps found)

KEGG Metabolic Maps

• Drug metabolism - other enzymes
• Pantothenate and CoA biosynthesis
• Pyrimidine metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.2.2

Use Curated BLAST to search for 3.5.2.2

Protein Sequence (484 amino acids)

>ABIE40_RS14535 dihydropyrimidinase (Rhizobium sp. OAE497)
MSTVIKGGTIVTADLTYKADVKIEGGKIVEIGPNLSGNEVLDASGCYVMPGGIDPHVHLE
MPFMGTYSSDDFESGTRAALAGGTTMVVDFCLPDPGQSLLDALKRWDNKSTRANCDYSFH
MSVTWWGEQVFNDMKTIVQEKGINTFKHFMAYKGALMVNDDEMFASFQRCAELGALPLVH
AENGDVVAAMQQKLMDEGNNGPEGHAYSRPASVEGEATNRAIIIADMAGVPLYVVHTSCE
QAHEAIRRARQNGMRVYGEPLIQHLTLDESEYFNRDWDHSARRVMSPPFRNKQHQDSLWA
GLSAGSLQVVATDHCAFTTDQKRTGLGDFRKIPNGTGGLEDRMPMLWTYGVVTGRITMNE
FVAVTSTNIAKILNVCPKKGAILVGADADIVVWDPKRSKTISAKSQQSAIDYNVFEGKEV
TGLPRYTLTRGVVAIEESTIKTQEGHGQFVKREPFQAVNKALSTWKELTAPRKVERTGIP
ASGV