Protein Info for ABIE40_RS11035 in Rhizobium sp. OAE497

Annotation: NADP-dependent phosphogluconate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 476 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details TIGR00873: 6-phosphogluconate dehydrogenase (decarboxylating)" amino acids 5 to 469 (465 residues), 615.8 bits, see alignment E=2.6e-189 PF03446: NAD_binding_2" amino acids 5 to 170 (166 residues), 162 bits, see alignment E=1.9e-51 PF03807: F420_oxidored" amino acids 6 to 104 (99 residues), 24.8 bits, see alignment E=4.1e-09 PF00393: 6PGD" amino acids 180 to 469 (290 residues), 381.9 bits, see alignment E=3.3e-118

Best Hits

Swiss-Prot: 46% identical to 6PGD_BUCAP: 6-phosphogluconate dehydrogenase, decarboxylating (gnd) from Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)

KEGG orthology group: K00033, 6-phosphogluconate dehydrogenase [EC: 1.1.1.44] (inferred from 92% identity to rec:RHECIAT_CH0002570)

Predicted SEED Role

"6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44)" in subsystem Pentose phosphate pathway (EC 1.1.1.44)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.44

Use Curated BLAST to search for 1.1.1.44

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (476 amino acids)

>ABIE40_RS11035 NADP-dependent phosphogluconate dehydrogenase (Rhizobium sp. OAE497)
MEKAEIGLIGLAVMGSNLALNIAEKGNKIAVFNRTPEKTDEFYESAGDLKKQIIPCKTIE
EFVEAIRPPRPIIIMIKAGEAVDQQMELLRPYLSKGDIMIDAGNANFRDTIARFDRLKNT
DLTFIGMGVSGGEEGARHGPSIMVGGTEDSWKRVEKVLTSIAAKYKGDPCVAWLGNDGAG
HFVKTIHNGIEYADMQMIAEIYGIFRDGLGKNSQEISKIFGQWNKGRLNSYLIEISEKVL
AATDPISKGSMVDMILDKAGQKGTGKWSAIEAQNMGIPATAIEAAVAARSISSMKDQREA
AEKIFGKPDYKFPIAYGPELDKDLELALFAAKIGAYAQGFAVMAEASREFNWSLPMPTIA
KIWRDGCIIRSQFLDEITSAFTKAPDAANLIVTPAFSEMVKESIPALRRVVTAALQAGLP
VPALTSALTYFDAYRQGRGTANLIQAQRDFFGAHGFDRLDGKDFHHGPWGSGAATF