Protein Info for ABIE40_RS10230 in Rhizobium sp. OAE497

Annotation: tRNA 2-thiocytidine(32) synthetase TtcA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 294 PF01171: ATP_bind_3" amino acids 61 to 226 (166 residues), 53.5 bits, see alignment E=1.3e-18

Best Hits

Swiss-Prot: 90% identical to TTCA_RHIL3: tRNA-cytidine(32) 2-sulfurtransferase (ttcA) from Rhizobium leguminosarum bv. viciae (strain 3841)

KEGG orthology group: K14058, tRNA 2-thiocytidine biosynthesis protein TtcA (inferred from 90% identity to rec:RHECIAT_CH0002406)

MetaCyc: 55% identical to tRNA cytosine32 2-sulfurtransferase TtcA (Escherichia coli K-12 substr. MG1655)
2.8.1.M2 [EC: 2.8.1.M2]

Predicted SEED Role

"tRNA(Cytosine32)-2-thiocytidine synthetase"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.M2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (294 amino acids)

>ABIE40_RS10230 tRNA 2-thiocytidine(32) synthetase TtcA (Rhizobium sp. OAE497)
MNIAANIADALDETTDDGGIAHALFADAPRSVSFNKLRKRLLRNVRQAFDDFDMLKGQKR
WLIGVSGGKDSYGLLALLLDLQWRGLLPVELVACNLDQGQPNFPKHILPEYLSGIGVKHR
IEYRDTYSIVKEKVPEGATYCSLCSRLRRGNLYRIAKEEGCDALVLGHHREDILETFFMN
FFHGGRLASMPAKLLNDEGNLMVLRPLAYCAEDDMAKFASAMQFPIIPCDLCGSQDGLQR
NAMKDMLADIERRMPGRKDTMLRALAHVNPSHLLDPKLFDFSSLMVTEPSSKSE