Protein Info for ABIE40_RS10095 in Rhizobium sp. OAE497
Annotation: flavin reductase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 42% identical to RUTF_STAND: FMN reductase (NADH) RutF (rutF) from Starkeya novella (strain ATCC 8093 / DSM 506 / CCM 1077 / IAM 12100 / NBRC 12443 / NCIB 9113)
KEGG orthology group: K13786, cob(II)yrinic acid a,c-diamide reductase [EC: 1.16.8.1] (inferred from 77% identity to rlg:Rleg_2123)MetaCyc: 54% identical to cob(II)yrinate a,c-diamide reductase monomer (Brucella melitensis)
Cob(I)yrinic acid a,c-diamide adenosyltransferase. [EC: 2.5.1.17]
Predicted SEED Role
"4-hydroxyphenylacetate 3-monooxygenase, reductase component (EC 1.6.8.-)" in subsystem 4-Hydroxyphenylacetic acid catabolic pathway or Aromatic Amin Catabolism (EC 1.6.8.-)
MetaCyc Pathways
- adenosylcobalamin biosynthesis II (aerobic) (30/33 steps found)
- adenosylcobinamide-GDP biosynthesis from cobyrinate a,c-diamide (6/6 steps found)
- adenosylcobalamin salvage from cobalamin (5/5 steps found)
- adenosylcobinamide-GDP salvage from cobinamide I (5/5 steps found)
- superpathway of adenosylcobalamin salvage from cobinamide I (7/8 steps found)
- adenosylcobinamide-GDP salvage from cobinamide II (5/6 steps found)
- superpathway of adenosylcobalamin salvage from cobinamide II (7/9 steps found)
- adenosylcobalamin biosynthesis I (anaerobic) (25/36 steps found)
- cobalamin salvage (eukaryotic) (4/8 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.6.8.-, 2.5.1.17
Use Curated BLAST to search for 1.16.8.1 or 1.6.8.- or 2.5.1.17
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (164 amino acids)
>ABIE40_RS10095 flavin reductase (Rhizobium sp. OAE497) MLSRQPIDPKLYRDAMSRFGGHVQLVTTALGDQVRGVTITAACSVSDSPACVLVCLNNSN PKNEIFFRSGIFALNTLGAHHQGLADAFSGRTQLSNEERFATGKFEKLVTGAPVLSDSLA SFDCRVMEIKEMSTHHVIFGEVVGVRFDETKPALLYMNRDYHTL