Protein Info for ABIE40_RS09850 in Rhizobium sp. OAE497

Annotation: acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00289: Biotin_carb_N
  • amino acids 1 to 110 (110 residues), 152.3 bits, E=2.6e-48
• PF02786: CPSase_L_D2
  • amino acids 115 to 322 (208 residues), 269.6 bits, E=6.9e-84
• PF07478: Dala_Dala_lig_C
  • amino acids 144 to 291 (148 residues), 39.3 bits, E=2.1e-13
• PF02785: Biotin_carb_C
  • amino acids 336 to 447 (112 residues), 118.8 bits, E=4.9e-38
• PF18140: PCC_BT
  • amino acids 467 to 594 (128 residues), 100.5 bits, E=3.1e-32
• PF00364: Biotin_lipoyl
  • amino acids 605 to 667 (63 residues), 48 bits, E=3.6e-16
• PF13533: Biotin_lipoyl_2
  • amino acids 606 to 640 (35 residues), 24.8 bits, see alignment (E = 6e-09)

Best Hits

KEGG orthology group: K01965, propionyl-CoA carboxylase alpha chain [EC: 6.4.1.3] (inferred from 87% identity to ara:Arad_2508)

Predicted SEED Role

"Biotin carboxylase (EC 6.3.4.14) / Biotin carboxyl carrier protein" (EC 6.3.4.14)

MetaCyc Pathways

• biotin-carboxyl carrier protein assembly (4/4 steps found)
• propanoyl CoA degradation I (3/3 steps found)
• 2-oxobutanoate degradation I (3/4 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (7/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
• 3-hydroxypropanoate cycle (7/13 steps found)
• superpathway of L-methionine salvage and degradation (9/16 steps found)
• methylaspartate cycle (11/19 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• glyoxylate assimilation (4/13 steps found)
• superpathway of the 3-hydroxypropanoate cycle (7/18 steps found)
• mycolate biosynthesis (26/205 steps found)
• superpathway of mycolate biosynthesis (27/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis
• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14, 6.4.1.3

Use Curated BLAST to search for 6.3.4.14 or 6.4.1.3

Protein Sequence (669 amino acids)

>ABIE40_RS09850 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha (Rhizobium sp. OAE497)
MFKKILIANRGEIACRVIKTARRMGILTVAVYSDADRDALHVEMADEAVHIGAAPAAESY
LVAEKIIAACKETGAEAVHPGYGFLSERASFCEALEKEGIVFIGPKPKAILAMGDKIESK
KFANAAKVSTVPGYLGVIEDAVQAEKIAGEIGYPVMIKASAGGGGKGMRIAWNEAEVHDG
FDRARSEAKSSFGDDRVFIEKYVVDPRHIEIQVLADGHGNAVYLGERECSIQRRNQKVAE
EAPSPFLDEKTRKAMGEQSIALAKAVDYQSAGTVEFIVDRDRNFYFLEMNTRLQVEHPVT
ELVTGIDLVEQMIRVAAGEKLPFAQKDIKLNGWAIESRLYAEDPYRNFLPSIGRLTRYRP
PAEGRHGDIIIRNDTGVYEGAEISMYYDPMIAKLCTWAPDRLTAVDAMAEALDAFVVDGI
EHNVPFLSALMKHPRWREGRLSTGFIAEEYPDGFAPVEPDASQKAALAAIALSASLIETN
RRERHGDRLRAATGDLRSDWVVKVGGDYVAASLLEGHVTIPFEMGMKADGRELHVVTNWR
PGEPVWSGTIDGVKVIAQIRPVLNGLRINWQGLSVRAKVFTPRHAELDRLMPVKLPPDTS
KLLLCPMPGLVVSIAVAEGQEVKAGETLAVVEAMKMENVLRAERDLVVGKVKAKPGESLA
VDAVIMEFA