Protein Info for ABIE40_RS09700 in Rhizobium sp. OAE497

Annotation: threonine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 668 PF02824: TGS" amino acids 8 to 62 (55 residues), 29.5 bits, see alignment 1.3e-10 TIGR00418: threonine--tRNA ligase" amino acids 74 to 648 (575 residues), 669.2 bits, see alignment E=3.1e-205 PF07973: tRNA_SAD" amino acids 172 to 221 (50 residues), 51.3 bits, see alignment 1.8e-17 PF00587: tRNA-synt_2b" amino acids 336 to 548 (213 residues), 167.7 bits, see alignment E=5.7e-53 PF03129: HGTP_anticodon" amino acids 560 to 647 (88 residues), 69.7 bits, see alignment E=3.9e-23

Best Hits

Swiss-Prot: 92% identical to SYT_RHIE6: Threonine--tRNA ligase (thrS) from Rhizobium etli (strain CIAT 652)

KEGG orthology group: K01868, threonyl-tRNA synthetase [EC: 6.1.1.3] (inferred from 92% identity to rec:RHECIAT_CH0002285)

Predicted SEED Role

"Threonyl-tRNA synthetase (EC 6.1.1.3)" (EC 6.1.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (668 amino acids)

>ABIE40_RS09700 threonine--tRNA ligase (Rhizobium sp. OAE497)
MSQSVSLTFPDGNVREFAAGTTGKDVAESISKSLAKKSVAIAIDGVVRDLSDPVVDGKIE
IITRSDERALELIRHDAAHVMAEAVQELWPGTQVTIGPVIENGFYYDFAKNEPFTPDDLP
KIEKKMKEIIARNAPFTKQVWSREKAKEVFAAKGEQYKVELVDAIPEGQDLKIYYQGDWF
DLCRGPHMASTGQIGTAFKLMKVAGAYWRGDSNNAMLSRIYGTAFAEQSELDNYLHILAE
AEKRDHRKLGREMDLFHFQEEGPGVVFWHGKGWRMFQSLTAYMRRRLAGTYEEVNAPQVL
DASLWETSGHWGWYQENMFAVKSAYAFTHPEDKEADNRIFALKPMNCPGHVQIFKHGLKS
YRELPIRLAEFGNVHRYEASGALHGLMRVRGFTQDDAHVFCTDEQMAAECLRINDLILSV
YEDFGFKEIVVKLSTRPEKRVGTDALWDRAESVMMDVLKTIEAQSEGRIKTGILPGEGAF
YGPKFEYTLKDAIGREWQCGTTQVDFNLPERFGAFYIDSNSEKTQPVMIHRAICGSMERF
LGILIENFAGHMPLWIAPQQIVVATITSEADGYGQEVADALRDAGLNVSTDFRNEKINYK
VREHSVTKVPVIIVCGKKEAEERTVNIRRLGSQEQTSMTLDAAIESLTWEATPPDVRRKI
EAKKAKAA