Protein Info for ABIE40_RS09100 in Rhizobium sp. OAE497

Annotation: sigma-54 dependent transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 454 PF00072: Response_reg" amino acids 5 to 114 (110 residues), 96.8 bits, see alignment E=2.7e-31 PF00158: Sigma54_activat" amino acids 142 to 305 (164 residues), 172.5 bits, see alignment E=1.9e-54 PF14532: Sigma54_activ_2" amino acids 143 to 310 (168 residues), 76.3 bits, see alignment E=8.4e-25 PF07728: AAA_5" amino acids 166 to 275 (110 residues), 22.9 bits, see alignment E=2.2e-08 PF02954: HTH_8" amino acids 412 to 450 (39 residues), 44.9 bits, see alignment 2.4e-15

Best Hits

KEGG orthology group: K13599, two-component system, NtrC family, nitrogen regulation response regulator NtrX (inferred from 96% identity to rlg:Rleg_1817)

Predicted SEED Role

"Nitrogen regulation protein NR(I)" in subsystem Ammonia assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (454 amino acids)

>ABIE40_RS09100 sigma-54 dependent transcriptional regulator (Rhizobium sp. OAE497)
MASDILVVDDEHDIREIVSGILSDEGHETRTAHDSDSALAAISDRAPRLIFLDIWMQGSK
LDGLSLLDEIKTRHPDIPVVMISGHGNIETAVSAIKRGAFDFIEKPFKADRLILIAERAL
ENSKLKREVSELKKRAGDAVELIGTSVAVSQLRQTIEKVSPTNSRIMIFGASGSGKELVA
RMIHKKSTRANGPFVALNAANITPDRMEIALFGTEGSPGQARKIGALEEAHRGILYLDEV
GEMPRETQNKILRVLVDQQFERVGGSKRVKVDVRIISSTAYNLESRIAEGWFREDLYHRL
AVVPVRVPALAERREDIPFLVDQLMRQISEQAGIRQRRIGDDAMAVLQAHDWPGNIRQLR
NNIERLMILARSDGPDAPITADMLPTDLGDMLPKVSAKNDYHIMTLPLREAREMFEKDYL
IAQINRFGGNISRTAEFVGMERSALHRKLKSLGV