Protein Info for ABIE40_RS07100 in Rhizobium sp. OAE497

Annotation: endopeptidase La

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 805 PF02190: LON_substr_bdg" amino acids 13 to 203 (191 residues), 189.1 bits, see alignment E=2.8e-59 TIGR00763: endopeptidase La" amino acids 14 to 772 (759 residues), 991.8 bits, see alignment E=1.5e-302 PF08298: AAA_PrkA" amino acids 322 to 374 (53 residues), 29.1 bits, see alignment 1.6e-10 PF07728: AAA_5" amino acids 354 to 486 (133 residues), 39.8 bits, see alignment E=1.4e-13 PF00004: AAA" amino acids 354 to 491 (138 residues), 84.4 bits, see alignment E=3e-27 PF05362: Lon_C" amino acids 571 to 773 (203 residues), 332.1 bits, see alignment E=3.7e-103 PF13541: ChlI" amino acids 620 to 741 (122 residues), 35.2 bits, see alignment E=3.2e-12

Best Hits

Swiss-Prot: 89% identical to LON_RHIME: Lon protease (lon) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01338, ATP-dependent Lon protease [EC: 3.4.21.53] (inferred from 94% identity to ara:Arad_1832)

MetaCyc: 64% identical to Lon protease (Escherichia coli K-12 substr. MG1655)
Endopeptidase La. [EC: 3.4.21.53]

Predicted SEED Role

"ATP-dependent protease La (EC 3.4.21.53) Type I" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent (EC 3.4.21.53)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.21.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (805 amino acids)

>ABIE40_RS07100 endopeptidase La (Rhizobium sp. OAE497)
MTKKTSVASSVAYPVLPLRDIVVFPHMIVPLFVGREKSIRALEEVMGSDKQIMLVTQINA
SDDDPDPSAIHNVGTVANVLQLLKLPDGTVKVLVEGRARAEIDAYTSREDFYEALGHVLE
EPHDDPVELEALSRSVVSEFESYVKLNKKISPEVVGAASQIDDYSKLADTVASHLSIKIT
EKQEMLETTSVKARLEKALGFMEGEISVLQVEKRIRSRVKRQMEKTQREYYLNEQMKAIQ
KELGDGEEGRDEMAELEERISKTKLSKEAREKADAELKKLRQMSPMSAEATVVRNYLDWL
LGIPWGKKSKIKSDLNNAEKILEADHFGLEKVKERIVEYLAVQARSTKIKGPILCLVGPP
GVGKTSLAQSIAKATGREYVRMALGGVRDEAEIRGHRRTYIGSMPGKVIQSMKKAKKSNP
LFLLDEIDKMGQDFRGDPSSALLEVLDPAQNSTFMDHYLEVEYDLSDVMFITTANTLNIP
APLMDRMEVIRIAGYTEDEKREIAKRHLLPKAIKEHALQPNEFSVSDDALMAISQQYTRE
AGVRSFERELMKLARKAVTEIIKGKTKSVHVTAENIDTYLGVPRFRHGEAEREDQVGVVT
GLAWTEVGGELLTIEGVMMPGKGRMTVTGNLKDVMKESISAAASYVRSRAVDFGIEPPRF
DKSDIHVHVPEGATPKDGPSAGVAMATAIVSIMTGIPVDKNVAMTGEITLRGRVLPIGGL
KEKLLAALRGGIKKVLIPEENAKDLAEIPDNVKNNMEIIPVSRMGEVIKHALIRRPEPIE
WDGTVETPVIATVEGLDDTGAGIAH