Protein Info for ABIE40_RS04515 in Rhizobium sp. OAE497

Annotation: choline dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 549 PF00732: GMC_oxred_N" amino acids 4 to 292 (289 residues), 196.4 bits, see alignment E=8.5e-62 TIGR01810: choline dehydrogenase" amino acids 4 to 531 (528 residues), 955.7 bits, see alignment E=3.4e-292 PF05199: GMC_oxred_C" amino acids 384 to 521 (138 residues), 144.8 bits, see alignment E=4.1e-46

Best Hits

Swiss-Prot: 91% identical to BETA_RHIEC: Oxygen-dependent choline dehydrogenase (betA) from Rhizobium etli (strain CFN 42 / ATCC 51251)

KEGG orthology group: K00108, choline dehydrogenase [EC: 1.1.99.1] (inferred from 91% identity to ret:RHE_CH01136)

MetaCyc: 89% identical to choline dehydrogenase (Sinorhizobium meliloti Rm2011)
Choline dehydrogenase. [EC: 1.1.99.1]

Predicted SEED Role

"Choline dehydrogenase (EC 1.1.99.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.1.99.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.1

Use Curated BLAST to search for 1.1.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (549 amino acids)

>ABIE40_RS04515 choline dehydrogenase (Rhizobium sp. OAE497)
MQADFVIIGSGSAGSALAYRLSEDGKNTVIVIEAGGSDFGPFIQMPAALAWPMSMKRYNW
GYLSEPEPNLNNRQITAPRGKVIGGSSSINGMVYVRGHAEDYNRWEELGAQGWAYSDVLP
YFKRMEHSHGGEEGWRGTDGPLHVQRGVFRNPLFQAFVEAGKQAGFETTEDYNGSKQEGF
GLMEQTIFGGRRWSAASAYLKPALKRPNVSIVYGLARKVVIENGRAVGVEIERGNSVEVI
KANREVIVSASSFNSPKLLMLSGIGPGAHLQEMGIEVKADRPGVGANLQDHMEFYFQQVS
TKPVSLYSWLPWFWQGVAGAQWMFTKSGLGTSNQFEACAFLRSAPGIKQPDIQYHFLPVA
ISYDGKAAAKSHGFQVHVGYNLSKSRGNVTLRSSDPKADPVIRFNYMSHPEDWEKFRHCV
RLTREIFGQQAFDQYRGPEIQPGEKVQSDDEIDTFLREHLESAYHPCGTCKMGAANDPIA
VVDPETRVIGVDNLRVADSSIFPHVTYGNLNGPSIMTGEKAADHILGKQPLPRSNQEPWV
NPRWAVSDR