Protein Info for ABIE40_RS01555 in Rhizobium sp. OAE497

Annotation: urocanate hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 553 TIGR01228: urocanate hydratase" amino acids 14 to 547 (534 residues), 699.7 bits, see alignment E=1.4e-214 PF17391: Urocanase_N" amino acids 16 to 135 (120 residues), 150.1 bits, see alignment E=4.1e-48 PF01175: Urocanase" amino acids 138 to 346 (209 residues), 271.6 bits, see alignment E=6.9e-85 PF17392: Urocanase_C" amino acids 349 to 542 (194 residues), 260.3 bits, see alignment E=1.4e-81

Best Hits

Swiss-Prot: 52% identical to HUTU_NATTJ: Urocanate hydratase (hutU) from Natranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF)

KEGG orthology group: K01712, urocanate hydratase [EC: 4.2.1.49] (inferred from 95% identity to rlg:Rleg_4631)

MetaCyc: 50% identical to HutU (Bacillus subtilis)
Urocanate hydratase. [EC: 4.2.1.49]

Predicted SEED Role

"Urocanate hydratase (EC 4.2.1.49)" in subsystem Histidine Degradation (EC 4.2.1.49)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.49

Use Curated BLAST to search for 4.2.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (553 amino acids)

>ABIE40_RS01555 urocanate hydratase (Rhizobium sp. OAE497)
MPKANPRHPKFPIPGGPELRAKGWRQEALLRLLENVLSVGEDPDNLIVYAALGKAARNWA
AHKGIVKALTEMDEDQTLLIQSGKPIGLVKTHAKAPLVIMANCNIVGQWAKAEVFYELQR
KGLICWGGLTAGAWQYIGSQGVIQGTYEIFMRIAERRFGGDLSGRFVLTAGLGGMGGAQP
LAGRMAGAAILCVDIDAERAKKRQEIGYLQEIAPDVDTALKMIDDAVKQKRALSVGLVGN
AAEIYPEIARRGIVPDVVTDQTSAHDLVYGYVPKGMSIAQVKELRDDGQGQLMAASRASI
VQHVTAMLDFQKKGSEVFDNGNLIRTQAKEGGVQNAFDIPIFTEAYLRPLFARAIGPFRW
MALSGEESDIARIDDLLLEMFPDNKIITNWIRLAREHVPFEGLPARIAWLGHGERTALAR
RVNELVASGELKGPVAFSRDHLDAGAMAHPNIMTERMKDGSDAIADWPLIDAMMLCSSMA
DLVVVHSGGGGYAGYMTSCGVTVVADGTVDADERLDHALTNDTALGVMRYADAGYEDALD
EVAKKDVPYIRLD