Protein Info for ABIE40_RS01150 in Rhizobium sp. OAE497

Annotation: phenylalanine--tRNA ligase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 811 TIGR00472: phenylalanine--tRNA ligase, beta subunit" amino acids 1 to 810 (810 residues), 707.1 bits, see alignment E=1.5e-216 PF01588: tRNA_bind" amino acids 44 to 149 (106 residues), 68.2 bits, see alignment E=1.4e-22 PF03483: B3_4" amino acids 219 to 390 (172 residues), 226 bits, see alignment E=6.1e-71 PF03484: B5" amino acids 409 to 473 (65 residues), 72.4 bits, see alignment E=7.2e-24 PF17759: tRNA_synthFbeta" amino acids 488 to 696 (209 residues), 137.4 bits, see alignment E=1.3e-43 PF03147: FDX-ACB" amino acids 717 to 810 (94 residues), 83.5 bits, see alignment E=3e-27

Best Hits

Swiss-Prot: 86% identical to SYFB_AGRFC: Phenylalanine--tRNA ligase beta subunit (pheT) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K01890, phenylalanyl-tRNA synthetase beta chain [EC: 6.1.1.20] (inferred from 90% identity to rec:RHECIAT_CH0000300)

Predicted SEED Role

"Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (811 amino acids)

>ABIE40_RS01150 phenylalanine--tRNA ligase subunit beta (Rhizobium sp. OAE497)
MKFTLSWLKDHLETDATLDEICTRLTEIGLEVEDVDDKAAYKPFVIARVLSAEKHPEADR
LKVLSVDAGPDVNGGKPLQIVCGAPNARAGLVGALARPGDYVPGIDVTLGVGKIRGVESH
GMMCSEKELNMSDDHDGIIDLPADAPVGTSFAAYAHLDDPVIEINLTPNRPDCTSIYGIA
RDLAASGLGTLKTRPAPSFAVEGETPVKLTLDLDDEKLCPGFGLRIVRGVKNGPSPKWMQ
QRLLAIGLRPINSLVDITNYMTFDQGRPMHVFDAAKVKGNLTVRRAKDGETVLALDQREY
KLGSNNVVIADENGVESIGGIMGGEHSGCDENTVDVLIESALWDPMNIAKSGRGLGIITD
ARYRFERGVDPEYMIPGLERTTELVLELCGGKPAKAEVVGYKGYQPKVVDFPYAEVKRLT
GLEVSTEESNAILTRLGFKVSGSGERVSVAVPSWRPDVDGKADLVEEIMRIHGVDKIKPA
PLESHAAVNGRILTTLQIRTRVAKRALASRGMLEAVTWSFISEDQAKLFGGGSPALKLAN
PIAAEMSDMRPSLLPGLLTAAQRNADKGYGDVAIFEVSGTYENDRPEGQRRVAGGIRRGT
ATMAGAGRMWSNASKGGGKPVDVFEAKADALAVIEACGLPMGNIQIEQGGPEWYHPGRSG
TIKMGPKVILGYFGEFHPSTLEALDVSGALCGFEVYVDAMPEPKKKATRTKPALELSAFQ
AVKRDFAFVVDKSVEAGAILKAASGVDRKLITGVNVFDIFEGASLGEGKKSVAIEVQIQP
VERTLTDEDFEALTQKIIASVTKFTGGVLRS