Protein Info for ABIE40_RS00455 in Rhizobium sp. OAE497

Annotation: molecular chaperone DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 636 TIGR02350: chaperone protein DnaK" amino acids 3 to 600 (598 residues), 987.7 bits, see alignment E=9e-302 PF00012: HSP70" amino acids 4 to 601 (598 residues), 939.4 bits, see alignment E=7.5e-287 PF06723: MreB_Mbl" amino acids 117 to 372 (256 residues), 57.7 bits, see alignment E=9e-20

Best Hits

Swiss-Prot: 96% identical to DNAK_RHIEC: Chaperone protein DnaK (dnaK) from Rhizobium etli (strain CFN 42 / ATCC 51251)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 96% identity to ret:RHE_CH00145)

MetaCyc: 68% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (636 amino acids)

>ABIE40_RS00455 molecular chaperone DnaK (Rhizobium sp. OAE497)
MAKVIGIDLGTTNSCVAVMDGKDAKVIENAEGARTTPSMVAFSDDGERLVGQPAKRQAVT
NPTNTLFAVKRLIGRRYDDPTVEKDKSLVPFKIAKGDNGDAWVEAQNKGYSPAQISAMIL
QKMKETAESYLGEKVEKAVITVPAYFNDAQRQATKDAGRIAGLEVLRIINEPTAAALAYG
LDKKDGKTIAVYDLGGGTFDISVLEIGDGVFEVKSTNGDTFLGGEDFDMRLVEYLVTEFK
KDQGIDLKNDKLALQRLKEAAEKAKIELSSSQQTEINLPFITADASGPKHLTLKLTRAKL
ESLVDDLVQRTVAPCKAALKDAGVTAAEIDEVVLVGGMSRMPKVQEVVKQLFGKEPHKGV
NPDEVVALGAAIQAGVLQGDVKDVLLLDVTPLSLGIETLGGVFTRLIERNTTIPTKKSQT
FSTADDNQQAVTIRVSQGEREMAQDNKLLGQFDLVGLPPAPRGVPQIEVTFDIDANGIVQ
VSAKDKGTGKEQQIRIQASGGLSDADIEKMVKDAEANAEADKKRREAVEAKNQAESLVHS
TEKSLKDYGDKVSEADRTAISDAIAALKSSIEATEPDAEDIKAKTQTLMEVSMKLGQAIY
EAQQAEAGTADASAGDDNVVDADYEEVKDDDRKKSA