Protein Info for ABIE40_RS00335 in Rhizobium sp. OAE497

Annotation: translation initiation factor IF-2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 898 PF08364: IF2_assoc" amino acids 15 to 52 (38 residues), 31.4 bits, see alignment (E = 7.8e-11) TIGR00487: translation initiation factor IF-2" amino acids 315 to 897 (583 residues), 784.4 bits, see alignment E=7.9e-240 PF04760: IF2_N" amino acids 320 to 371 (52 residues), 42.9 bits, see alignment 1.5e-14 TIGR00231: small GTP-binding protein domain" amino acids 398 to 555 (158 residues), 113.2 bits, see alignment E=1.1e-36 PF09439: SRPRB" amino acids 398 to 526 (129 residues), 25.7 bits, see alignment E=3e-09 PF00009: GTP_EFTU" amino acids 400 to 556 (157 residues), 120.5 bits, see alignment E=3e-38 PF02421: FeoB_N" amino acids 401 to 554 (154 residues), 31.9 bits, see alignment E=4.2e-11 PF01926: MMR_HSR1" amino acids 401 to 506 (106 residues), 44.3 bits, see alignment E=7.9e-15 PF00071: Ras" amino acids 402 to 557 (156 residues), 27.3 bits, see alignment E=1.1e-09 PF11987: IF-2" amino acids 675 to 788 (114 residues), 142 bits, see alignment E=3.6e-45 PF03144: GTP_EFTU_D2" amino acids 819 to 886 (68 residues), 36.8 bits, see alignment 2e-12

Best Hits

Swiss-Prot: 83% identical to IF2_RHIL3: Translation initiation factor IF-2 (infB) from Rhizobium leguminosarum bv. viciae (strain 3841)

KEGG orthology group: K02519, translation initiation factor IF-2 (inferred from 80% identity to ara:Arad_0173)

Predicted SEED Role

"Translation initiation factor 2" in subsystem NusA-TFII Cluster or Translation initiation factors eukaryotic and archaeal or Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (898 amino acids)

>ABIE40_RS00335 translation initiation factor IF-2 (Rhizobium sp. OAE497)
MTDSNDDKTLGATGKKTLTLKPSGISQGTVRQDMGRGRTKAVVVETRKRRPMRPEDERPV
APAPAAPRAAEPAPAPVQARPQPQAPAPRIQQPSSQQQAPRQQDRPRPVVLNHLSPEEMD
ARRRALAMAQVREAEDAARRVEEEKRRAVEEAARQAAEAEEAARRAAEEAARPPAPPVVE
APAPAPAAEPVQARAAQPSAPAARRPDAAPAPGAARPGSAAAPAAARGRREGEEDDRSGA
ARGAPARGRVVRPEPAKPVTTRPKGEEDRRRGKLTIGTAGVDDEGARGRSMAATRRRQEK
FRRSQMQETREKVIREVILPETITIQELSQRMSERAVDVIKYLMKEGQMMKPGDVIDADL
AELIASEFGHTVKRVSESDVEEGIFDKADDAGEMVSRPPVVTIMGHVDHGKTSLLDAIRQ
ANVVSGEAGGITQHIGAYQVEQNGQKITFIDTPGHAAFTAMRARGAQATDIAILVVAADD
SVMPQTIESINHAKAAGVPIIVAINKIDKHEAKPDRVRQQLLQHEVFVESLGGEVLDVEV
SAKNKTNLDKLLEAVLLQAEILDLKADPTRTAEGTVIEAQLDRGRGAVATVLVQKGTLKP
GQIIVAGDQWGRVRALVTDKGDHVKEAGPATPVEVLGLSGTPAAGDKFAVVENEARAREI
SEYRQRLARDKAAARQSGQRGSLEQMMSKLQNTGYKEFPLVIKGDVQGSIEAIIGALDKL
GTDEVRARIVHSGAGAITESDISLAESSDAAIIGFNVRANAQARTAAERGGIEIRYYNII
YDLVDDVKAAMSGLLSPERRETFLGNAEILEVFNITKTGKVAGCRVTEGKVERGAGVRLI
RDNVVIHEGKLKTLKRFKDEVSEVPVGQECGMAFENYEDIRAGDVIECFRVEHITRSL