Protein Info for ABID97_RS28960 in Variovorax sp. OAS795

Annotation: AMP-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 27 to 402 (376 residues), 239.2 bits, E=6.8e-75
• PF13193: AMP-binding_C
  • amino acids 452 to 527 (76 residues), 77.1 bits, E=1.7e-25

Best Hits

KEGG orthology group: K00666, fatty-acyl-CoA synthase [EC: 6.2.1.-] (inferred from 91% identity to vap:Vapar_6221)

MetaCyc: 49% identical to cinnamate:CoA ligase (Hypericum calycinum)
4-coumarate--CoA ligase. [EC: 6.2.1.12]; 6.2.1.- [EC: 6.2.1.12]

Predicted SEED Role

"3-methylmercaptopropionyl-CoA ligase (DmdB)"

MetaCyc Pathways

• 4-coumarate degradation (aerobic) (5/5 steps found)
• 4-hydroxybenzoate biosynthesis III (plants) (5/5 steps found)
• 4-coumarate degradation (anaerobic) (5/6 steps found)
• umbelliferone biosynthesis (2/3 steps found)
• 5,6-dehydrokavain biosynthesis (engineered) (7/10 steps found)
• cinnamoyl-CoA biosynthesis (1/2 steps found)
• 6-gingerol analog biosynthesis (engineered) (3/6 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (5/9 steps found)
• benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (2/5 steps found)
• trans-caffeate degradation (aerobic) (1/4 steps found)
• naringenin biosynthesis (engineered) (1/4 steps found)
• xanthohumol biosynthesis (1/4 steps found)
• chlorogenic acid biosynthesis II (1/5 steps found)
• flavonoid biosynthesis (1/5 steps found)
• phaselate biosynthesis (1/5 steps found)
• rosmarinic acid biosynthesis I (3/10 steps found)
• chlorogenic acid biosynthesis I (1/8 steps found)
• avenanthramide biosynthesis (1/9 steps found)
• superpathway of rosmarinic acid biosynthesis (4/14 steps found)
• curcuminoid biosynthesis (1/10 steps found)
• coumarins biosynthesis (engineered) (3/13 steps found)
• flavonoid di-C-glucosylation (3/15 steps found)
• monolignol biosynthesis (1/15 steps found)
• suberin monomers biosynthesis (3/20 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis II
• Benzoate degradation via CoA ligation
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Caprolactam degradation
• Ethylbenzene degradation
• Geraniol degradation
• Limonene and pinene degradation
• Phenylpropanoid biosynthesis
• Propanoate metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.-

Use Curated BLAST to search for 6.2.1.- or 6.2.1.12

Protein Sequence (550 amino acids)

>ABID97_RS28960 AMP-binding protein (Variovorax sp. OAS795)
MSAQSYAQGLERCEANHVPLTPLGFLDRAALAHPNRPAVVHGELSRTWEQTRERCLRLAS
ALVARGILPGDTVSVLAPNTPAMLESHFGVPLSGAVLNAINHRLDPEGIAFILRHGECKL
LMVDREFSGTVAAALELLDRPPVVVDIEDHLAPPGERIGETDYESLLASGDPAFAGRWPD
DEWQPIALNYTSGTTGDPKGVVASHRGTYLMSLLQITNWAMPRSPRYLWTLPMFHANGWC
FTWAVTAAAGTHVCLRRVSAEAVMEAIDRHGIDHLCAAPVVMAMLADSARHTRLSRAVRV
LTAGSPPPLSVLNAVLAMGFDVEHVFGITEVCGTPVSCVWQDGWDDLPQAEQGKLRVRQG
VRAAMFEGLMVGDADTLAPVPADGTAAGELMLRGNTVMMGYFKNEAATRKALAGGWFRTG
DVAVLHGNGYAQITDRSKDVIISGGENISSVEVEDVVHGHPAVLHAAVVAQPDEKWGEVP
CVFIELRAGVPAPTEQEIVAFCRERLAHFKCPRRVVFSSLPKTATGKIQKFRLREQAGSR
EAIVQRPNHD