Protein Info for ABID97_RS28310 in Variovorax sp. OAS795

Annotation: alpha/beta hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00561: Abhydrolase_1
  • amino acids 27 to 128 (102 residues), 57.1 bits, E=3.3e-19
• PF12697: Abhydrolase_6
  • amino acids 29 to 255 (227 residues), 64.5 bits, E=3.5e-21
• PF12146: Hydrolase_4
  • amino acids 33 to 223 (191 residues), 43.2 bits, E=4.5e-15

Best Hits

Swiss-Prot: 56% identical to NICD_PSEPK: N-formylmaleamate deformylase (nicD) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: None (inferred from 93% identity to vap:Vapar_4284)

MetaCyc: 54% identical to N-formylmaleamate deformylase (Alcaligenes faecalis)
RXN-11318 [EC: 3.5.1.106]

Predicted SEED Role

"Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase" in subsystem cAMP signaling in bacteria

MetaCyc Pathways

• picolinate degradation (6/7 steps found)
• nicotinate degradation I (4/6 steps found)
• nicotine degradation II (pyrrolidine pathway) (5/11 steps found)
• nicotine degradation III (VPP pathway) (4/10 steps found)
• superpathway of nicotinate degradation (8/18 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.106

Protein Sequence (268 amino acids)

>ABID97_RS28310 alpha/beta hydrolase (Variovorax sp. OAS795)
MGRFLHGYHVRANGVRLHLLRYGGQGPRLLLLPGITSPAITWGFVAERLARHFDVHVLDF
RGRGLSFSAPGMDASLDAMAADVLDLLGVLGWSSVTVLGHSMGARVAIRAAALDPGRMAR
LLLVDPPMSGPGRRPYPVPLSWYVESIQMAVSGITAEELRPYAPTWTDEQVQLRAEWLHT
CDQGAIVQAHAGFHKDDIHADLPKLQTPGLLMAAGRGGVMLPEDLAEIEALAPGLLQCTA
LNAGHMIPWDDEEDFHRLLGEYLKVDLR