Protein Info for ABID97_RS28060 in Variovorax sp. OAS795

Annotation: 2-oxoacid:acceptor oxidoreductase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03710: 2-oxoacid:acceptor oxidoreductase, alpha subunit
  • amino acids 12 to 597 (586 residues), 580.1 bits, E=2.4e-178
• PF01558: POR
  • amino acids 15 to 188 (174 residues), 113 bits, E=1.6e-36
• PF01855: POR_N
  • amino acids 224 to 390 (167 residues), 165.6 bits, E=1.7e-52

Best Hits

KEGG orthology group: K00174, 2-oxoglutarate ferredoxin oxidoreductase subunit alpha [EC: 1.2.7.3] (inferred from 94% identity to vap:Vapar_5478)

Predicted SEED Role

"2-oxoglutarate oxidoreductase, alpha subunit (EC 1.2.7.3)" (EC 1.2.7.3)

MetaCyc Pathways

• TCA cycle V (2-oxoglutarate synthase) (9/9 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• TCA cycle VII (acetate-producers) (7/9 steps found)
• reductive TCA cycle I (8/11 steps found)
• incomplete reductive TCA cycle (5/7 steps found)
• reductive TCA cycle II (7/12 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (24/56 steps found)

KEGG Metabolic Maps

• Citrate cycle (TCA cycle)
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 1.2.7.3

Use Curated BLAST to search for 1.2.7.3

Protein Sequence (618 amino acids)

>ABID97_RS28060 2-oxoacid:acceptor oxidoreductase subunit alpha (Variovorax sp. OAS795)
MSAVPQIEAVNDFVIKFANVNGSGSASANELFAKAILRMGVPVSPRNIFPSNIQGLPTWY
EVRVTEAGHLGRRGGTDMMVAMNPQTWDADLAELEPGGYLFYDSTRPLPPSKFRSDIRVI
GMPLTEICNAVYQDPRQRQLFKNIVYVGALAILLGIEPQVIEALFGEQYKGKEKLLASNV
QALHLGCDFARENLRESLGLQVRRADRVGDRIFVDGNSAAALGCVYGGATVAAWYPITPS
SSVAEAFQKYCAKFRVDPATGQHRFAIVQAEDELASIGVVVGAGWNGARAFTATSGPGIS
LMAEFIGLAYFAEIPVTLINVQRGGPSTGMPTRTQQADILACAYASHGDTKHVLLFPEDP
HECFEHAAAALDLADRLQTPVFVMTDLDIGMNQRLCAPFDWDDAKAYDRGKVMSAEELEA
GRDFGRYKDVDGDGIPWRTLPGTHPSKGSYFTRGTTRDAYARYSERGPDYIYNMERLLKK
FATAARLVPQPVLRPAAEETALGVIYFGSTSPAMHEALQALEARGIHLDALRLRAFPFPD
SVARFLARHAKVFVVEQNRDAQMRSLLVNELDIDPARLVKVLHFDGTPITARFIAGAIGS
HLQAQASGAATTHTGEPA