Protein Info for ABID97_RS27590 in Variovorax sp. OAS795

Annotation: L-glutamate gamma-semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01237: putative delta-1-pyrroline-5-carboxylate dehydrogenase
  • amino acids 6 to 515 (510 residues), 717.2 bits, E=6e-220
• PF00171: Aldedh
  • amino acids 54 to 512 (459 residues), 474.6 bits, E=1.5e-146

Best Hits

Swiss-Prot: 55% identical to ROCA_GEOTN: 1-pyrroline-5-carboxylate dehydrogenase (rocA) from Geobacillus thermodenitrificans (strain NG80-2)

KEGG orthology group: K00294, 1-pyrroline-5-carboxylate dehydrogenase [EC: 1.5.1.12] (inferred from 64% identity to mrb:Mrub_2639)

Predicted SEED Role

"Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)" in subsystem Arginine and Ornithine Degradation or Proline, 4-hydroxyproline uptake and utilization (EC 1.5.1.12)

MetaCyc Pathways

• L-arginine degradation I (arginase pathway) (2/3 steps found)
• L-proline degradation I (2/3 steps found)
• ethene biosynthesis II (microbes) (1/4 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.5.1.12

Use Curated BLAST to search for 1.5.1.12

Protein Sequence (516 amino acids)

>ABID97_RS27590 L-glutamate gamma-semialdehyde dehydrogenase (Variovorax sp. OAS795)
MHLLPYANEPYDTYTTPASLEAMATALRSVRAQFGQTYPMVIGGKKVMSEALLVSTNPSA
PSEVVGKSARATREHAEAALDAAWTAFESWKRWKQEDRSRVMLKAAAIMRRRKQELEAWL
VYEIGKNYVEASAEVAEMIDFIEYYARQAMRHVGGLGHLNAYAGEENESFYIPLGAGLTI
SPWNFPMALMTGMTVGAIVVGNTVVCKPAEDTVVSLAKVFDIFEEAGLPPGVVNYLPGVG
AEVGSYLVEHPRTRFINFTGSLATGKRINEAAAKVVDGQRWFKRVFLELGGKDAILVDET
ADLDAAALGVIQGAYGYQGQKCSACSRLIVVDSVYDALLERVVERTKKLSLAPADQNPDL
GPVCNAAQDAKVREYLEIGHSEGKLLVGGQRLDGEGFFIQPTVFGDVAPGARLAQEEVFG
PVVAAIRVKNFTEGMAVVNNTVYGLTGALFSRDRSRIEIARRDFHVGNLYFNRKCTGALA
GVQPFGGFNLTGTDTKTGGPDYLLQFMQMKSVAERL