Protein Info for ABID97_RS27395 in Variovorax sp. OAS795

Annotation: 5-oxoprolinase subunit PxpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03746: LamB_YcsF
  • amino acids 3 to 240 (238 residues), 311.3 bits, E=2.4e-97

Best Hits

Swiss-Prot: 65% identical to PXPA2_RALSO: 5-oxoprolinase subunit A 2 (pxpA2) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K07160, UPF0271 protein (inferred from 93% identity to vap:Vapar_6367)

MetaCyc: 44% identical to 5-oxoprolinase component A (Escherichia coli K-12 substr. MG1655)
5-oxoprolinase (ATP-hydrolyzing). [EC: 3.5.2.9]

Predicted SEED Role

"Lactam utilization protein LamB"

MetaCyc Pathways

• 5-oxo-L-proline metabolism (5/6 steps found)
• γ-glutamyl cycle (4/6 steps found)

KEGG Metabolic Maps

• Glutathione metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.2.9

Use Curated BLAST to search for 3.5.2.9

Protein Sequence (254 amino acids)

>ABID97_RS27395 5-oxoprolinase subunit PxpA (Variovorax sp. OAS795)
MDIDLNADLGEGFGPWRMGEDEALLDIVSSANVACGFHAGDPVIMDRTVRMALARGVDVG
AHVGFPDLLGFGRRPMQIEPEELVACVLYQLSALDGMARTAGHRMTHMSFHGALGNMAAA
DAALAEPLVRAVADFNPALIISSSASRAIEDAAARSGLRVRTTFLADRACGDDGLLVPRK
LPDAVIHDRGAVLARVRQLLEQGTVTSYSGREIPMRAHSILLHGDTPGAVELARAVREVV
EQAGRVVPISRQRD