Protein Info for ABID97_RS25255 in Variovorax sp. OAS795

Annotation: glycine--tRNA ligase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 710 transmembrane" amino acids 584 to 603 (20 residues), see Phobius details TIGR00211: glycine--tRNA ligase, beta subunit" amino acids 1 to 708 (708 residues), 601.5 bits, see alignment E=1.6e-184 PF02092: tRNA_synt_2f" amino acids 6 to 572 (567 residues), 621.4 bits, see alignment E=1.7e-190 PF05746: DALR_1" amino acids 602 to 698 (97 residues), 31.1 bits, see alignment E=2.2e-11

Best Hits

Swiss-Prot: 72% identical to SYGB_DECAR: Glycine--tRNA ligase beta subunit (glyS) from Dechloromonas aromatica (strain RCB)

KEGG orthology group: K01879, glycyl-tRNA synthetase beta chain [EC: 6.1.1.14] (inferred from 95% identity to vap:Vapar_4743)

Predicted SEED Role

"Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (710 amino acids)

>ABID97_RS25255 glycine--tRNA ligase subunit beta (Variovorax sp. OAS795)
MPSANLLVELFVEELPPKALKKLGEAFAGGLRDQLVAQGLAAPGAVLTAYASPRRLAAHL
THVADRAADKAVSQKLMPVAVGLDASGQPTPALLKRLAALGADASAVPGLKRAVDGKAEA
LFYESTAKGATLAEGLQKALAEAIAKLPIPKVMSYQLETGCELPGWSSVSFVRPAHGLVA
LHGSEVVPVEALGLHAGRETHGHRFEAAIDPVVLRDANSYALQMQDDGAVIASFAARRTE
IARQLAAAAVQVGGGTVPIHDDALLDEVTALVERPNVLVCSFEREFLEVPQECLILTMKA
NQKYFPLLDASNRLTNKFLVVSNISPDDASAVVGGNERVVRPRLADAKFFFDQDRKKSLA
SRVESLGKVVYHNKLGTQGERVERVMRIARAIAAHLGDATLTAHAVQAAQLAKADLVTDM
VGEFPELQGTMGRYYALHDGLAADVADAIEDHYKPRFAGDGLPRHKVGIVVALADKLETL
VGMFGIGNLPTGDRDPFALRRHALGVIRMLIEKDLPLKLDALLQDTAAQFKDIDGFDSGK
ATAELQDFILDRLAGSLREQGASALEVDAVLAPRPQRLGEVPKLLAAVRAFAALPAAAAL
AAANKRIGNILKKAPEADAHVSELLLHEPAEKALHAAMAEVVPAANAQFDAGDYTASLQT
LAALREPVDAFFDGVMVNAEQADLRLNRLGLLMSLHVAMNRVAQLERLAA