Protein Info for ABID97_RS25195 in Variovorax sp. OAS795

Annotation: acetyl-CoA C-acyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00108: Thiolase_N
  • amino acids 5 to 264 (260 residues), 289.6 bits, E=2.2e-90
• TIGR01930: acetyl-CoA C-acyltransferase
  • amino acids 7 to 392 (386 residues), 423 bits, E=5.2e-131
• PF02803: Thiolase_C
  • amino acids 272 to 392 (121 residues), 150 bits, E=2.4e-48

Best Hits

Swiss-Prot: 49% identical to THIL1_DICDI: Probable acetyl-CoA acetyltransferase (DDB_G0271544) from Dictyostelium discoideum

KEGG orthology group: K00626, acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] (inferred from 98% identity to vap:Vapar_4732)

MetaCyc: 64% identical to acetyl-CoA C-acetyltransferase (Methylibium petroleiphilum PM1)
Acetyl-CoA C-acyltransferase. [EC: 2.3.1.16, 2.3.1.9]

Predicted SEED Role

"3-ketoacyl-CoA thiolase (EC 2.3.1.16)" in subsystem Biotin biosynthesis or Isoleucine degradation or Polyhydroxybutyrate metabolism or Serine-glyoxylate cycle or n-Phenylalkanoic acid degradation (EC 2.3.1.16)

MetaCyc Pathways

• oleate β-oxidation (29/35 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (21/26 steps found)
• (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (5/5 steps found)
• 4-hydroxybenzoate biosynthesis III (plants) (5/5 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• ketolysis (3/3 steps found)
• polyhydroxybutanoate biosynthesis (3/3 steps found)
• fatty acid salvage (5/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
• ketogenesis (4/5 steps found)
• 2-deoxy-D-ribose degradation II (6/8 steps found)
• (2S)-ethylmalonyl-CoA biosynthesis (3/4 steps found)
• acetyl-CoA fermentation to butanoate (5/7 steps found)
• fatty acid β-oxidation I (generic) (5/7 steps found)
• 2-methyl-branched fatty acid β-oxidation (10/14 steps found)
• 5,6-dehydrokavain biosynthesis (engineered) (7/10 steps found)
• L-isoleucine degradation I (4/6 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• acetoacetate degradation (to acetyl CoA) (1/2 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• pyruvate fermentation to butanoate (4/7 steps found)
• propanoate fermentation to 2-methylbutanoate (3/6 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (5/9 steps found)
• valproate β-oxidation (5/9 steps found)
• glycerol degradation to butanol (10/16 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
• 3-phenylpropanoate degradation (5/10 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
• 4-ethylphenol degradation (anaerobic) (2/6 steps found)
• ethylbenzene degradation (anaerobic) (1/5 steps found)
• fatty acid β-oxidation VII (yeast peroxisome) (1/5 steps found)
• isopropanol biosynthesis (engineered) (1/5 steps found)
• pyruvate fermentation to acetone (1/5 steps found)
• pyruvate fermentation to butanol I (3/8 steps found)
• mevalonate pathway I (eukaryotes and bacteria) (2/7 steps found)
• mevalonate pathway II (haloarchaea) (2/7 steps found)
• 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) (4/10 steps found)
• superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) (4/10 steps found)
• 10-trans-heptadecenoyl-CoA degradation (MFE-dependent, yeast) (1/6 steps found)
• 4-oxopentanoate degradation (3/9 steps found)
• L-glutamate degradation VII (to butanoate) (5/12 steps found)
• 2-methylpropene degradation (2/8 steps found)
• isoprene biosynthesis II (engineered) (2/8 steps found)
• mevalonate pathway III (Thermoplasma) (2/8 steps found)
• mevalonate pathway IV (archaea) (2/8 steps found)
• L-tryptophan degradation III (eukaryotic) (7/15 steps found)
• ethylmalonyl-CoA pathway (4/11 steps found)
• L-lysine fermentation to acetate and butanoate (3/10 steps found)
• methyl tert-butyl ether degradation (3/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (8/17 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (5/13 steps found)
• 10-cis-heptadecenoyl-CoA degradation (yeast) (2/12 steps found)
• 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) (2/12 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (5/17 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (4/16 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
• jasmonic acid biosynthesis (5/19 steps found)
• toluene degradation VI (anaerobic) (4/18 steps found)
• cholesterol degradation to androstenedione I (cholesterol oxidase) (2/17 steps found)
• Spodoptera littoralis pheromone biosynthesis (4/22 steps found)
• sitosterol degradation to androstenedione (1/18 steps found)
• androstenedione degradation I (aerobic) (6/25 steps found)
• platensimycin biosynthesis (6/26 steps found)
• superpathway of ergosterol biosynthesis I (6/26 steps found)
• cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (3/22 steps found)
• superpathway of testosterone and androsterone degradation (7/28 steps found)
• androstenedione degradation II (anaerobic) (4/27 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (24/56 steps found)
• superpathway of L-lysine degradation (10/43 steps found)
• superpathway of cholesterol biosynthesis (6/38 steps found)
• superpathway of cholesterol degradation I (cholesterol oxidase) (8/42 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (9/47 steps found)
• superpathway of cholesterol degradation III (oxidase) (5/49 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Benzoate degradation via hydroxylation
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Ethylbenzene degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Lysine degradation
• Propanoate metabolism
• Pyruvate metabolism
• Synthesis and degradation of ketone bodies
• Terpenoid biosynthesis
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.16, 2.3.1.9

Use Curated BLAST to search for 2.3.1.16 or 2.3.1.9

Protein Sequence (394 amino acids)

>ABID97_RS25195 acetyl-CoA C-acyltransferase (Variovorax sp. OAS795)
MSESIVIVGAARTPMGSFQGDFSSLSAHDLGGAAIKAAVERAGIAPDSVGEVLFGNCLMA
GQGQAPARQAAYKGGLPDSAGAVTLSKMCGSAMKAAMLSHDLLLAGTHDVMVAGGMESMT
NAPYLMLKGRGGYRMGHDRIFDHMMLDGLEDAYQPGRSMGTFGEDCAAKYKFTREQQDAF
AIASVERAKAATESGAFKDEITPVTVKGRGGDTVISIDEGPGKVKLDKIATLKPAFKKEN
GTITAASSSSINDGAAALVMMTESHAKKIGARPIARIVGHATHAQQPEWFSTAPVGAVAK
LFKKTGWTVKDVDLWEVNEAFAVVPMALMHELDVSHDIVNVNGGACALGHPIGASGARIM
VTLIHALKARGKKRGVATLCIGGGEGTAVALELI