Protein Info for ABID97_RS24220 in Variovorax sp. OAS795

Annotation: NAD-dependent epimerase/dehydratase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01370: Epimerase
  • amino acids 2 to 232 (231 residues), 153.5 bits, E=2.4e-48
• PF04321: RmlD_sub_bind
  • amino acids 2 to 223 (222 residues), 29.9 bits, E=1.1e-10
• PF01073: 3Beta_HSD
  • amino acids 2 to 222 (221 residues), 63.4 bits, E=6.2e-21
• PF05368: NmrA
  • amino acids 2 to 116 (115 residues), 23.2 bits, E=1.5e-08
• PF16363: GDP_Man_Dehyd
  • amino acids 3 to 294 (292 residues), 128 bits, E=2.1e-40
• PF13460: NAD_binding_10
  • amino acids 3 to 149 (147 residues), 38.2 bits, E=5e-13
• PF02719: Polysacc_synt_2
  • amino acids 46 to 255 (210 residues), 20.5 bits, E=8.3e-08

Best Hits

KEGG orthology group: K01784, UDP-glucose 4-epimerase [EC: 5.1.3.2] (inferred from 42% identity to cpb:Cphamn1_2145)

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (11/11 steps found)
• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• UDP-α-D-galactofuranose biosynthesis (1/2 steps found)
• D-galactose degradation I (Leloir pathway) (3/5 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
• D-galactose detoxification (1/3 steps found)
• stachyose degradation (2/7 steps found)
• UDP-sugars interconversion (3/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (1/18 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 5.1.3.2

Protein Sequence (308 amino acids)

>ABID97_RS24220 NAD-dependent epimerase/dehydratase family protein (Variovorax sp. OAS795)
MLGGRGFLGRNLVDLLLRDGASVRTFDRPLASAADQPVFEGDVEEFEGNFIDGQGLEAAL
KNVDLVYHLISTTVPSGSNADPIGDVTSNLSGTLRLLKAMSAAGVDRIVYASSGGTVYGN
PCVLPVPEWHALQPISSYGVVKVAVENYLHLHSELNGLKANVLRISNPYGMHQRRIGAQG
VIATFLDKLMNRKPIEIWGDGSVVRDYIYVADVARALLLAGRREKSGTFNIGSGIGHSLN
DVLRVLQQQTGLETEVRYMPHRKFDVSQTFLDIERAREELGWNPFYTLEEGCARFCQLME
RAREPALL