Protein Info for ABID97_RS22330 in Variovorax sp. OAS795

Annotation: 2-hydroxy-3-oxopropionate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 302 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF03807: F420_oxidored" amino acids 6 to 68 (63 residues), 28 bits, see alignment E=4.1e-10 TIGR01505: 2-hydroxy-3-oxopropionate reductase" amino acids 6 to 299 (294 residues), 384.6 bits, see alignment E=1.4e-119 PF03446: NAD_binding_2" amino acids 6 to 168 (163 residues), 153 bits, see alignment E=1.1e-48 PF14833: NAD_binding_11" amino acids 171 to 291 (121 residues), 139.6 bits, see alignment E=8.8e-45

Best Hits

Swiss-Prot: 43% identical to GARR_ECOLI: 2-hydroxy-3-oxopropionate reductase (garR) from Escherichia coli (strain K12)

KEGG orthology group: K00042, 2-hydroxy-3-oxopropionate reductase [EC: 1.1.1.60] (inferred from 98% identity to vap:Vapar_3956)

MetaCyc: 43% identical to tartronate semialdehyde reductase (Escherichia coli K-12 substr. MG1655)
2-hydroxy-3-oxopropionate reductase. [EC: 1.1.1.60]

Predicted SEED Role

"2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60)" in subsystem Allantoin Utilization or D-galactarate, D-glucarate and D-glycerate catabolism or Photorespiration (oxidative C2 cycle) (EC 1.1.1.60)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.60

Use Curated BLAST to search for 1.1.1.60

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (302 amino acids)

>ABID97_RS22330 2-hydroxy-3-oxopropionate reductase (Variovorax sp. OAS795)
MSQSQKIGFIGLGIMGAPMAGHLLDAGHQLFVNTQGNTPEPFISKATICASAAEVARLAD
VIFIMVPDTPDVEKVLFGEPGTQGVAEGLKDSCGKIVVDCSSIDPIATKAFAKRILALGC
GYIDAPVSGGEVGAKAASLTIMCGGDEGTFGQVRPLLEKMGKNVTLVGNVGDGQVCKVAN
QIIVALNIAAVGEALLFASKAGADPAKVRQALMGGFASSRILEVHGERMIKRTFAPGFRI
SLHQKDLNLALQSARALGVALPQTAGAAQLMNACAALGHGQQDHSALVRALEALAQHTVT
PD