Protein Info for ABID97_RS21630 in Variovorax sp. OAS795

Annotation: O-succinylhomoserine sulfhydrylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01053: Cys_Met_Meta_PP
  • amino acids 14 to 401 (388 residues), 429.7 bits, E=1.2e-132
• TIGR01325: O-succinylhomoserine sulfhydrylase
  • amino acids 14 to 401 (388 residues), 539 bits, E=2.3e-166
• PF00155: Aminotran_1_2
  • amino acids 57 to 191 (135 residues), 25.9 bits, E=8.4e-10
• PF01041: DegT_DnrJ_EryC1
  • amino acids 64 to 190 (127 residues), 20.8 bits, E=3e-08

Best Hits

Swiss-Prot: 45% identical to METZ_MYCTO: O-succinylhomoserine sulfhydrylase (metZ) from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

KEGG orthology group: K10764, O-succinylhomoserine sulfhydrylase [EC: 2.5.1.-] (inferred from 96% identity to vap:Vapar_3758)

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48, EC 2.5.1.49)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (8/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (8/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (7/8 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• L-homocysteine biosynthesis (2/2 steps found)
• L-methionine biosynthesis I (4/5 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (4/6 steps found)
• homocysteine and cysteine interconversion (2/4 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (1/5 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (1/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Carotenoid biosynthesis - General
• Cysteine metabolism
• Methionine metabolism
• Porphyrin and chlorophyll metabolism
• Riboflavin metabolism
• Selenoamino acid metabolism
• Sulfur metabolism
• Terpenoid biosynthesis
• Tryptophan metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.-, 2.5.1.48, 2.5.1.49

Use Curated BLAST to search for 2.5.1.- or 2.5.1.48 or 2.5.1.49

Protein Sequence (409 amino acids)

>ABID97_RS21630 O-succinylhomoserine sulfhydrylase (Variovorax sp. OAS795)
MTDERTLPPGLHRDTLALRTGLAPSQHGEHSEALFLTSGFVQPDAETSARRFAGTEAGFT
YTRTSNPTVTSFEQRLAAMEGTEAAIGASTGMGAILMMCMGLLKAGDHVVCSRSVFGSTL
NLFGKEFAKFGVETTFVSQTDVAEWRAALKPNTRLLFAETPTNPLTEVCDIRALADLAHG
AGALLAVDNCFCTPVLQRPTELGADLVIHSGTKYLDGQGRVMAGAICGPSKLIVDVFGPV
VRTAGMALSPFNAWVVLKGLETLGIRMQAQCANALAVAQWLEAQPGVARVYYPGLASHAQ
HDLAMRQQSGQGGAVVSFDVVGDNPETARANAFHVINSTRVVSIATNLGDTKTIVTHPGT
TSHGRLTEAQRQAAGISQGLIRVAVGLEYIDDIKADLSRGLGGLGTLKS