Protein Info for ABID97_RS20965 in Variovorax sp. OAS795

Annotation: glutamate--cysteine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 518 transmembrane" amino acids 175 to 194 (20 residues), see Phobius details TIGR01434: glutamate--cysteine ligase" amino acids 16 to 498 (483 residues), 498.2 bits, see alignment E=1.3e-153 PF04262: Glu_cys_ligase" amino acids 16 to 361 (346 residues), 460.6 bits, see alignment E=3.1e-142 PF04107: GCS2" amino acids 51 to 187 (137 residues), 22.4 bits, see alignment E=7.1e-09

Best Hits

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 93% identity to vap:Vapar_3637)

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.2.2

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (518 amino acids)

>ABID97_RS20965 glutamate--cysteine ligase (Variovorax sp. OAS795)
MSSRLQERLGAIPTTRLKGMRRGIEKESLRALPDGKLALTPHPIALGSALTHPHITTDFS
ESQLELITGVHGGVESALEELTRVHQFTYRVLDTLGDERLWVSSMPCGLPTDETIPIGRY
GSSNVGRAKSVYRMGLSHRYGRRMQTISGIHYNWSLPDVSSEQYFALIRNFRRHAFLLLY
LFGASPALCSSFVAGRPHELQPLGDGSMFMPHGTSLRMGRLGYQSDAQASLAVSYNSLEG
YAASLQDALTRPWPGYEAIGIQNLGGDYNQLATSLLQIENEFYGTIRPKRVINPGERPLH
ALRERGVEYVEVRLMDLDPFEPVGINAQTMRFLDVFLLQCLLSDSPVDTPQEIAELAHNQ
HLTAACGREPGLKLLRSGREVPLTDWGVELVEQCLPIAAALDAANGGTQHADAVRAALSA
LQDPDSLPSARVLAAIEERHGNSFIGFVRAQSEQTRTTLLALPFSDQQKAEFERMTEESI
QEQKRIEAADTMPFEIYREQYVSPARLGIGRGRMAVAA