Protein Info for ABID97_RS20330 in Variovorax sp. OAS795

Annotation: threo-3-hydroxy-L-aspartate ammonia-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00291: PALP
  • amino acids 18 to 305 (288 residues), 263.7 bits, E=1.1e-82

Best Hits

Swiss-Prot: 71% identical to LTHAD_PSESP: L-threo-3-hydroxyaspartate ammonia-lyase (thadh) from Pseudomonas sp.

KEGG orthology group: K01754, threonine dehydratase [EC: 4.3.1.19] (inferred from 94% identity to vap:Vapar_3470)

MetaCyc: 71% identical to L-threo-3-hydroxy aspartate ammonia-lyase (Pseudomonas sp. T62)
Threo-3-hydroxyaspartate ammonia-lyase. [EC: 4.3.1.16]

Predicted SEED Role

"Threonine dehydratase, catabolic (EC 4.3.1.19)" in subsystem Branched-Chain Amino Acid Biosynthesis or Glycine and Serine Utilization or Threonine anaerobic catabolism gene cluster or Threonine degradation (EC 4.3.1.19)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• superpathway of L-threonine metabolism (15/18 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• L-methionine degradation II (3/3 steps found)
• L-threonine degradation I (5/6 steps found)
• L-threonine degradation V (1/2 steps found)
• hypoglycin biosynthesis (4/14 steps found)
• cyclosporin A biosynthesis (2/15 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.19

Use Curated BLAST to search for 4.3.1.16 or 4.3.1.19

Protein Sequence (318 amino acids)

>ABID97_RS20330 threo-3-hydroxy-L-aspartate ammonia-lyase (Variovorax sp. OAS795)
MQLPTYDDVIAAAARLEGHAHRTPVLRSRTADERWGAGFFFKCENFQRMGAFKFRGAFNA
LSRFDAAQRKGGVIAFSSGNHAQAIALSARLLSMPAVIVMPNDAPAAKVAATRGYGAEVV
MYDRFAEDREALTRRLAVERGMTMIPPYDHPDVLAGQGTAVKELIEEAGPLDQLFVCLGG
GGLLSGSALSARALAPGCKIYGVEPEAGNDGQQSLRAGKIVHIATPKTIADGAQTQHLGE
YTFGIIRRDVDDIFTVTDAQLVEAMRFFAERMKIVVEPTGCLAFAGAIAAGKAIAGQRVG
IVVSGGNVDLSRYAALLA