Protein Info for ABID97_RS20320 in Variovorax sp. OAS795

Annotation: tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00174: tRNA dimethylallyltransferase
  • amino acids 17 to 304 (288 residues), 285.3 bits, E=2.5e-89
• PF01715: IPPT
  • amino acids 50 to 302 (253 residues), 284.2 bits, E=1e-88

Best Hits

Swiss-Prot: 69% identical to MIAA_POLNA: tRNA dimethylallyltransferase (miaA) from Polaromonas naphthalenivorans (strain CJ2)

KEGG orthology group: K00791, tRNA dimethylallyltransferase [EC: 2.5.1.75] (inferred from 88% identity to vap:Vapar_3468)

Predicted SEED Role

"tRNA dimethylallyltransferase (EC 2.5.1.75)" (EC 2.5.1.75)

MetaCyc Pathways

• cis-zeatin biosynthesis (1/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Zeatin biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.75

Protein Sequence (328 amino acids)

>ABID97_RS20320 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA (Variovorax sp. OAS795)
MPPSVAATGPPDTLKFIALAGPTASGKTAVALAVARVRPVEIVSVDSALVYRGMDIGTAK
PSLAEQAAVPHHLIDILDAAESYSAAAFVADATRLVGEIRARGALPLLVGGTMLYFKALF
DGIDAMPAADAAVRARIDAEAAERGWPAMHARLAEVDPATAARLAPQDSQRIQRALEVWE
SSGQPLSSFHARDKNHAAKGLAGGALFSLEPTDRGWLHARIAERFDAMLAAGFLDEVRAL
RARGDLSLQLPSMRCVGYRQAWEMLDACGSSAPDPRAMAELRERGIAATRQLAKRQITWL
RSMPARSVIACDAPDAVQTAVQRIATAE